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Noxa Recombinant Rabbit Monoclonal Antibody [JA30-03]

Usd: 385

Specification

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Catalog# ET1704-35

Noxa Recombinant Rabbit Monoclonal Antibody [JA30-03]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Noxa Recombinant Rabbit Monoclonal Antibody [JA30-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Noxa aa 5-54 / 54.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 6 kDa

Positive Control

Human spleen tissue, human liver tissue, Jurkat, mouse lung tissue, rat lung tissue.

Conjugation

unconjugated

Clone Number

JA30-03

RRID

AB_3070485

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:8,000

Target

Function

Members of the Bcl-2 family of proteins interact to regulate programmed cell death (apoptosis) under a broad range of physiological conditions. Bcl-2, Bcl-xL, and several related proteins inhibit apoptosis, whereas other members of the Bcl-2 family, such as Bax and Bak, enhance cell death. NOXA, a pro-apoptotic member of the Bcl-2 family, contains the Bcl-2 homology 3 (BH3) region, but does not contain other BH domains. Murine cells constitutively express NOXA mRNA in small amounts in various organs; X-ray irradiation increases NOXA mRNA and protein expression levels. In human cells, NOXA, alternatively designated PMA-induced protein 1 or APR, displays high expression in the adult T cell leukemia cell line IKD, where it may function as an immediate-early-response gene. The NOXA protein selectively localizes to mitochondria.

Background References

1. Zheng YJ. et. al. Silencing lncRNA LOC101928963 Inhibits Proliferation and Promotes Apoptosis in Spinal Cord Glioma Cells by Binding to PMAIP1. Mol Ther Nucleic Acids. 2019 Dec

2. Beyfuss K. et. al. A systematic review of p53 regulation of oxidative stress in skeletal muscle. Redox Rep. 2018 Dec

Sequence Similarity

Belongs to the PMAIP1 family.

Tissue Specificity

Highly expressed in adult T-cell leukemia cell line.

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: Q13794 Human

SwissProt: Q9JM54 Mouse

SwissProt: Q5U777 Rat

Synonyms

Adult T cell leukemia derived PMA responsive antibody

APR antibody

APR_HUMAN antibody

ATL-derived antibody

Immediate early response protein APR antibody

Immediate-early-response protein APR antibody

NOXA antibody

Phorbol 12 myristate 13 acetate induced protein 1 antibody

Phorbol-12-myristate-13-acetate-induced protein 1 antibody

PMA induced protein 1 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Noxa antibody (<a href="/products/ET1704-35" style="font-weight: bold;text-decoration: underline;">ET1704-35</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-35" style="font-weight: bold;text-decoration: underline;">ET1704-35</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Noxa antibody (ET1704-35) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-35) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Noxa antibody (<a href="/products/ET1704-35" style="font-weight: bold;text-decoration: underline;">ET1704-35</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-35" style="font-weight: bold;text-decoration: underline;">ET1704-35</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Noxa antibody (ET1704-35) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-35) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"