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Nicastrin Recombinant Rabbit Monoclonal Antibody [JG68-33]

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Specification

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Catalog# ET7108-38

Nicastrin Recombinant Rabbit Monoclonal Antibody [JG68-33]

Application

  • WB

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Nicastrin Recombinant Rabbit Monoclonal Antibody [JG68-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recomninant protein within Human Nicastrin aa 5-112 / 709.

Species Reactivity

Human, Mouse (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB

Molecular Weight

Predicted band size: 78 kDa

Positive Control

THP-1 cell lysates, NIH/3T3 cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate.

Conjugation

unconjugated

Clone Number

JG68-33

RRID

AB_3070837

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

The Presenilin 1 (PS1) and Presenilin 2 (PS2) transmembrane proteins are components of high molecular weight complexes. These complexes mediate proteolytic cleavage within the transmembrane domain of several proteins, including the β-Amyloid precursor protein (βAPP) and Notch. Missense mutations in the genes encoding the Presenilin proteins increase the proteolysis of βAPP and results in the overproduction of the neurotoxic β-Amyloid peptide, which results in a condition associated with Familial Alzheimer's disease (FAD). A novel component of the presenilin complex, nicastrin, is a type I transmembrane glycoprotein that is involved in mediating Notch/GLP-1 signaling. In addition, nicastrin contributes to the processing of βAPP, which makes nicastrin an attractive potential target for modulating the production of β-Amyloid in patients with Alzheimer's disease. Originally purified from immunoprecipitated PS1 complexes from HEK293 cells, nicastrin contains hydrophilic amino and carboxy-terminal domains, a short, hydrophobic transmembrane domain and potential N-myristoylation and phosphorylation sites.

Background References

1. Yu G et al. Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. Nature 407:48-54 (2000).

2. Lu P et al. Three-dimensional structure of human gamma-secretase. Nature 512:166-170 (2014).

Sequence Similarity

Belongs to the nicastrin family.

Tissue Specificity

Detected in brain (at protein level). Widely expressed.

Post-translational Modification

N-glycosylated.

Subcellular Location

Membrane, Cytoplasmic vesicle membrane, Melanosome.

UNIPROT

SwissProt: Q92542 Human

SwissProt: P57716 Mouse

Synonyms

Anterior pharynx defective 2 antibody

APH 2 antibody

APH2 antibody

ATAG1874 antibody

KIAA0253 antibody

Ncstn antibody

NCT antibody

NICA_HUMAN antibody

Nicastrin antibody

RP11 517F10.1 antibody

Expand

Images

  • Western blot analysis of Nicastrin on THP-1 cell lysates with Rabbit anti-Nicastrin antibody (<a href="/products/ET7108-38" style="font-weight: bold;text-decoration: underline;">ET7108-38</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 78-150 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-38" style="font-weight: bold;text-decoration: underline;">ET7108-38</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Nicastrin on THP-1 cell lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/1,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 78-150 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (<a href="/products/ET7108-38" style="font-weight: bold;text-decoration: underline;">ET7108-38</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 2: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 3: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 78 kDa<br />Observed band size: 78-150 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-38" style="font-weight: bold;text-decoration: underline;">ET7108-38</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 2: Neuro-2a cell lysate (20 µg/Lane)
    Lane 3: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 78 kDa
    Observed band size: 78-150 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (<a href="/products/ET7108-38" style="font-weight: bold;text-decoration: underline;">ET7108-38</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Nicastrin KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 78-150 kDa<br /><br />Exposure time: 60 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-38" style="font-weight: bold;text-decoration: underline;">ET7108-38</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Nicastrin KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 78-150 kDa

    Exposure time: 60 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation