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Mast Cell Chymase Recombinant Rabbit Monoclonal Antibody [JB74-32]

Usd: 385

Specification

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Catalog# ET7107-19

Mast Cell Chymase Recombinant Rabbit Monoclonal Antibody [JB74-32]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Mast Cell Chymase Recombinant Rabbit Monoclonal Antibody [JB74-32]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Mast Cell Chymase aa 198-247 / 247.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 27 kDa

Positive Control

Mouse spleen tissue lysate, Rat spleen tissue lysate, human lymph nodes tissue, human skin tissue, human tonsil tissue.

Conjugation

unconjugated

Clone Number

JB74-32

RRID

AB_3070734

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:200-1:20,000

Target

Function

Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast Cell Chymase, also known as CMA1 or MCT1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19 amino acid signal peptide, an acidic 2 amino acid propeptide and a 226 amino acid catalytic domain. Mast Cell Chymase is a chymotryptic serine proteinase which is a member of the peptidase family S1. Expressed in mast cells, Mast Cell Chymase is associated with the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function. Mast Cell Protease 1, also designated Mcp-1 or Mcpt1, is a rodent specific beta-chymase. The mouse and rat Mast Cell Protease 1 proteins share 76% sequence identity at the amino acid level.

Background References

1. Schechter N M et al. Determination of the primary structures of human skin chymase and cathepsin G from cutaneous mast cells of urticaria pigmentosa lesions. J Immunol 152:4062-4069 (1994).

2. Caughey G H et al. Structure, chromosomal assignment, and deduced amino acid sequence of a human gene for mast cell chymase. J Biol Chem 266:12956-12963 (1991).

Sequence Similarity

Belongs to the peptidase S1 family. Granzyme subfamily.

Tissue Specificity

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

Subcellular Location

Secreted, Cytoplasmic granule.

UNIPROT

SwissProt: P23946 Human

SwissProt: P21844 Mouse

SwissProt: P50339 Rat

Synonyms

Alpha-chymase antibody

Chymase 1 antibody

Chymase 1 mast cell antibody

chymase 1 preproprotein transcript E antibody

chymase 1 preproprotein transcript I antibody

Chymase antibody

Chymase, heart antibody

Chymase, mast cell antibody

CMA1 antibody

CMA1_HUMAN antibody

Expand

Images

  • Western blot analysis of Mast Cell Chymase on different lysates with Rabbit anti-Mast Cell Chymase antibody (<a href="/products/ET7107-19" style="font-weight: bold;text-decoration: underline;">ET7107-19</a>) at 1/500 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 2 minutes;<br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-19" style="font-weight: bold;text-decoration: underline;">ET7107-19</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Mast Cell Chymase on different lysates with Rabbit anti-Mast Cell Chymase antibody (ET7107-19) at 1/500 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

    Exposure time: 2 minutes;
    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-19) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Mast Cell Chymase antibody (<a href="/products/ET7107-19" style="font-weight: bold;text-decoration: underline;">ET7107-19</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-19" style="font-weight: bold;text-decoration: underline;">ET7107-19</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Mast Cell Chymase antibody (ET7107-19) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-19" style="font-weight: bold;text-decoration: underline;">ET7107-19</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-19" style="font-weight: bold;text-decoration: underline;">ET7107-19</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"