MAPKAP Kinase 2 Recombinant Rabbit Monoclonal Antibody [JU30-31]
Catalog# ET7106-83
MAPKAP Kinase 2 Recombinant Rabbit Monoclonal Antibody [JU30-31]
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WB
-
IP
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Human
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Mouse
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unconjugated
Overview
Product Name
MAPKAP Kinase 2 Recombinant Rabbit Monoclonal Antibody [JU30-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MAPKAP Kinase 2 aa 351-400 / 400.
Species Reactivity
Human, Mouse
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 46 kDa
Positive Control
SK-Br-3 cell lysates.
Conjugation
unconjugated
Clone Number
JU30-31
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IP
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1:10-1:50
Target
Function
The MAPKAP kinases (for MAP kinase activated protein kinases) are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. These include the serine/threonine kinases Rsk-1 (also designated MAPKAP kinase-1), Rsk-2 and Rsk-3, which are phosphorylated by ERK1 and ERK2. Similarly, p38 phosphorylates and activates the serine/threonine kinases MAPKAP kinase-2 and MAPKAP kinase-3 (also designated 3pK). The serine/threonine kinases Mnk1 and Mnk2 are substrates for both ERK and p38 MAP kinases.
Background References
1. Stokoe D et al. The substrate specificity and structure of mitogen-activated protein (MAP) kinase-activated protein kinase-2. Biochem J 296:843-849 (1993).
2. Jakob U et al. Small heat shock proteins are molecular chaperones. J Biol Chem 268:1517-1520 (1993).
Sequence Similarity
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Tissue Specificity
Expressed in all tissues examined.
Post-translational Modification
Sumoylation inhibits the protein kinase activity.; Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.
Subcellular Location
Cytoplasm. Nucleus.
Synonyms
MAP kinase activated protein Kinase 2 antibody
MAP kinase-activated protein kinase 2 antibody
MAPK activated protein kinase 2 antibody
MAPK-activated protein kinase 2 antibody
MAPK2_HUMAN antibody
MAPKAP K2 antibody
MAPKAP kinase 2 antibody
MAPKAPK 2 antibody
MAPKAPK-2 antibody
MAPKAPK2 antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of MAPKAP Kinase 2 on different lysates with Rabbit anti-MAPKAP Kinase 2 antibody (ET7106-83) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si MAPKAP Kinase 2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-83) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of MAPKAP Kinase 2 on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7106-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"