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Lipoprotein lipase Recombinant Rabbit Monoclonal Antibody [JA22-02]

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Specification

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Catalog# ET1704-48

Lipoprotein lipase Recombinant Rabbit Monoclonal Antibody [JA22-02]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Lipoprotein lipase Recombinant Rabbit Monoclonal Antibody [JA22-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Lipoprotein lipase aa 456-475 / 475.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 53 kDa

Positive Control

Human placenta tissue lysates, human ileum tissue, human pancreas tissue.

Conjugation

unconjugated

Clone Number

JA22-02

RRID

AB_3070495

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

Target

Function

The Lipase gene family belongs to one of the most robust genetic superfamilies found in living organisms, which includes esterases and thioesterases. Lipase gene products are related by tertiary structure rather than primary amino acid sequence. Members of the AB hydrolase subfamily include hepatic lipase (HL), endothelial lipase (EL), lipoprotein lipase (LPL) and pancreatic lipase (PL). HL balances the composition and transport of lipoproteins in human plasma. Synthesized in endothelial cells, EL hydrolyzes high density lipoproteins. LPL, a homodimer attached to the membrane by a GPI-anchor, mediates the hydrolysis of triglycerides of very low density lipoproteins and circulating chylomicrons. Defects in LPL may cause chylomicronemia syndrome or a form of lipoprotein lipase deficiency characterized by hypertriglyceridemia.

Background References

1. Bal NC et al. Increased Reliance on Muscle-based Thermogenesis upon Acute Minimization of Brown Adipose Tissue Function. J Biol Chem 291:17247-57 (2016).

2. Alfaradhi MZ et al. Oxidative stress and altered lipid homeostasis in the programming of offspring fatty liver by maternal obesity. Am J Physiol Regul Integr Comp Physiol 307:R26-34 (2014).

Sequence Similarity

Belongs to the AB hydrolase superfamily. Lipase family.

Tissue Specificity

Detected in blood plasma. Detected in milk (at protein level).

Post-translational Modification

Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

Subcellular Location

Cell membrane. Secreted.

UNIPROT

SwissProt: P06858 Human

Synonyms

EC 3.1.1 antibody

EC 3.1.1.34 antibody

HDLCQ11 antibody

LIPD antibody

LIPL_HUMAN antibody

Lipoprotein lipase antibody

LPL antibody

LPL protein antibody

MGC137861 antibody

Images

  • Western blot analysis of Lipoprotein lipase on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-48" style="font-weight: bold;text-decoration: underline;">ET1704-48</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Lipoprotein lipase on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human ileum tissue using anti-Lipoprotein lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-48" style="font-weight: bold;text-decoration: underline;">ET1704-48</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ileum tissue using anti-Lipoprotein lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Lipoprotein lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-48" style="font-weight: bold;text-decoration: underline;">ET1704-48</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Lipoprotein lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation