LMAN1 Recombinant Rabbit Monoclonal Antibody [JE55-19]
Catalog# ET7110-98
LMAN1 Recombinant Rabbit Monoclonal Antibody [JE55-19]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
LMAN1 Recombinant Rabbit Monoclonal Antibody [JE55-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human LMAN1 aa 281-405 / 510.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
58 kDa
Positive Control
NIH/3T3 cell lysate, human placenta tissue lysate, HUVEC cell lysate, rat brain tissue, mouse kidney tissue, mouse testis tissue.
Conjugation
unconjugated
Clone Number
JE55-19
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:50-1:200
Target
Function
The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined deficiency of factor V-factor VIII, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. MCFD2 is the second gene that leads to combined deficiency of factor V-factor VIII. ERGIC-53 and MCFD2 form a protein complex and serve as a cargo receptor to transport FV and FVIII from the ER to the ERGIC and then the Golgi, as illustrated here.
Background References
1. Fu YL. et. al. LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochem Biophys Res Commun. 2019 Apr
2. Zhu M. et. al. Analysis of MCFD2- and LMAN1-deficient mice demonstrates distinct functions in vivo. Blood Adv. 2018 May
Tissue Specificity
Ubiquitous.
Post-translational Modification
The N-terminal may be partly blocked.
Subcellular Location
Golgi apparatus membrane, endoplasmic reticulum membrane, endoplasmic reticulum-golgi intermediate compartment membrane.
Synonyms
Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
ER-Golgi intermediate compartment 53 kDa protein antibody
ERGIC-53 antibody
ERGIC53 antibody
ERGIC53 like protein antibody
F5F8D antibody
FMFD1 antibody
Gp58 antibody
Intracellular mannose specific lectin antibody
Intracellular mannose-specific lectin MR60 antibody
ExpandImages
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Western blot analysis of LMAN1 on different lysates with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 154 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7110-98, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 58 kDa
Observed band size: 58 kDa -
☑ Knockdown (KD)
Western blot analysis of LMAN1 on different lysates with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-LMAN1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 55 kDa
Exposure time: 60 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-98) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-98) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-98) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-98) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"