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Kallikrein 5 Recombinant Rabbit Monoclonal Antibody [JE54-94]

Usd: 385

Specification

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Catalog# ET7110-90

Kallikrein 5 Recombinant Rabbit Monoclonal Antibody [JE54-94]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Kallikrein 5 Recombinant Rabbit Monoclonal Antibody [JE54-94]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Kallikrein 5 aa 161-293 / 293.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 32 kDa

Positive Control

MCF-7 cell lysate, SK-Br-3 cell lysate, MCF-7, A431.

Conjugation

unconjugated

Clone Number

JE54-94

RRID

AB_3071049

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:100

  • FC

  • 1:50-1:100

Target

Function

Kallikrein-related peptidase 5 (KLK5), formerly known as stratum corneum tryptic enzyme (SCTE), is a serine protease expressed in the epidermis. In humans it is encoded by the KLK5 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. Alternative splicing results in multiple transcript variants encoding the same protein. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

Background References

1. Kasparek P. et. al. KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype. PLoS Genet. 2017 Jan.

2. Pampalakis G. et. al. KLK5, a novel potential suppressor of vaginal carcinogenesis. Biol Chem. 2018 Sep.

Sequence Similarity

Belongs to the peptidase S1 family. Kallikrein subfamily.

Tissue Specificity

Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.

Subcellular Location

Secreted.

UNIPROT

SwissProt: Q9Y337 Human

Synonyms

HK5 antibody

Kallikrein 5 antibody

Kallikrein L2 antibody

Kallikrein like 2 antibody

Kallikrein like protein 2 antibody

Kallikrein related peptidase 5 antibody

Kallikrein-5 antibody

Kallikrein-like protein 2 antibody

Kallikrein5 antibody

KLK 5 antibody

Expand

Images

  • Western blot analysis of Kallikrein 5 on different lysates with Rabbit anti-Kallikrein 5 antibody (<a href="/products/ET7110-90" style="font-weight: bold;text-decoration: underline;">ET7110-90</a>) at 1/500 dilution.<br /><br />Lane 1: MCF-7 cell lysate<br />Lane 2: SK-Br-3 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 32 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 2 minutes;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-90" style="font-weight: bold;text-decoration: underline;">ET7110-90</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Kallikrein 5 on different lysates with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/500 dilution.

    Lane 1: MCF-7 cell lysate
    Lane 2: SK-Br-3 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 32 kDa
    Observed band size: 40 kDa

    Exposure time: 2 minutes;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Rabbit anti-Kallikrein 5 antibody (<a href="/products/ET7110-90" style="font-weight: bold;text-decoration: underline;">ET7110-90</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Kallikrein 5 antibody (<a href="/products/ET7110-90" style="font-weight: bold;text-decoration: underline;">ET7110-90</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of Kallikrein 5 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET7110-90" style="font-weight: bold;text-decoration: underline;">ET7110-90</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Kallikrein 5 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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