IKB epsilon Recombinant Rabbit Monoclonal Antibody [JM62-63]
Catalog# ET1705-40
IKB epsilon Recombinant Rabbit Monoclonal Antibody [JM62-63]
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WB
-
IHC-P
-
IP
-
Human
-
unconjugated
Overview
Product Name
IKB epsilon Recombinant Rabbit Monoclonal Antibody [JM62-63]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human IKB epsilon aa 175-224 / 500.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 53 kDa
Positive Control
THP-1 cell lysate, Jurkat cell lysate, Hela cell lysate, human tonsil tissue.
Conjugation
unconjugated
Clone Number
JM62-63
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:200
Target
Function
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon, also known as NFKBIE, is a protein which in humans is encoded by the NFKBIE gene. NFKBIE protein expression is up-regulated following NF-κB activation and during myelopoiesis. NFKBIE is able to inhibit NF-κB-directed transactivation via cytoplasmic retention of REL proteins. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-κB transcription factor complex. The NF-κB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NF-κB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NF-κB interaction with other transcription factors, such as STAT (see STAT6), AP-1 (JUN), and NFAT (see NFATC1).
Background References
1. Kim, S.J. et al. Ligation of TLR5 Promotes Myeloid Cell Infiltration and Differentiation into Mature Osteoclasts in Rheumatoid Arthritis and Experimental Arthritis. Journal of immunology (Baltimore, Md. : 1950). 193: 3902-13 (2014).
2. King KE, et al. The p53 homologue DeltaNp63alpha interacts with the nuclear factor-kappaB pathway to modulate epithelial cell growth Cancer Res. 68(13): 5122-5131 (2008).
Sequence Similarity
Belongs to the NF-kappa-B inhibitor family.
Tissue Specificity
Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Post-translational Modification
Serine phosphorylated; followed by proteasome-dependent degradation.
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
I kappa B epsilon antibody
I-kappa-B-epsilon antibody
IkappaBepsilon antibody
IkB E antibody
IkB-E antibody
IkB-epsilon antibody
IKBE antibody
IKBE_HUMAN antibody
MGC72568 antibody
NF kappa B inhibitor epsilon antibody
ExpandImages
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Western blot analysis of IKB epsilon on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-40, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Predicted band size: 53 kDa
Observed band size: 40 kDa -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKB epsilon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"