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IGFBP2 Recombinant Rabbit Monoclonal Antibody [JU37-46]

Usd: 385

Specification

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Catalog# ET7106-63

IGFBP2 Recombinant Rabbit Monoclonal Antibody [JU37-46]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Green monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IGFBP2 Recombinant Rabbit Monoclonal Antibody [JU37-46]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal Human IGFBP2 .

Species Reactivity

Human, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 35 kDa

Positive Control

SH-SY5Y cell lysate, T-47D cell lysate, MCF7 cell lysate, BxPC-3 cell lysate, COS-1 cell lysate, human non-small cell lung carcinoma tissue, human tonsil tissue.

Conjugation

unconjugated

Clone Number

JU37-46

RRID

AB_3070683

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

The Insulin-like growth factor-binding proteins (IGFBPs), a family of homologous proteins that have co-evolved with the IGFs, serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system. Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, and their concentration and distribution in the extracellular environment. The IGFBPs also appear to have biological activity independent of the IGFs. Seven IGFBPs have been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors. IGFBP1 is negatively regulated by Insulin production. The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes. IGFBP2, which may function as a chaperone, escorting IGFs to their target tissues, is expressed in several human tissues including fetal eye and fetal brain.

Background References

1. Chin C et al. Loss of IGFBP2 mediates alveolar type 2 cell senescence and promotes lung fibrosis. Cell Rep Med. 2023 Mar

2. Lin YH et al. IGFBP2 expressing midlobular hepatocytes preferentially contribute to liver homeostasis and regeneration. Cell Stem Cell. 2023 May

Post-translational Modification

O-glycosylated.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P18065 Human

Synonyms

BP 2 antibody

BP2 antibody

IBP 2 antibody

IBP-2 antibody

IBP2 antibody

IBP2_HUMAN antibody

IGF binding protein 2 antibody

IGF BP53 antibody

IGF-binding protein 2 antibody

IGFBP 2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of IGFBP2 on different lysates with Rabbit anti-IGFBP2 antibody (<a href="/products/ET7106-63" style="font-weight: bold;text-decoration: underline;">ET7106-63</a>) at 1/5,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: T-47D cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: BxPC-3 cell lysate<br />Lane 5: JEG-3 cell lysate (low expression)<br />Lane 6: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7106-63" style="font-weight: bold;text-decoration: underline;">ET7106-63</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of IGFBP2 on different lysates with Rabbit anti-IGFBP2 antibody (ET7106-63) at 1/5,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: T-47D cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: BxPC-3 cell lysate
    Lane 5: JEG-3 cell lysate (low expression)
    Lane 6: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-63) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma tissue with Rabbit anti-IGFBP2 antibody (<a href="/products/ET7106-63" style="font-weight: bold;text-decoration: underline;">ET7106-63</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-63" style="font-weight: bold;text-decoration: underline;">ET7106-63</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma tissue with Rabbit anti-IGFBP2 antibody (ET7106-63) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-63) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IGFBP2 antibody (<a href="/products/ET7106-63" style="font-weight: bold;text-decoration: underline;">ET7106-63</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-63" style="font-weight: bold;text-decoration: underline;">ET7106-63</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IGFBP2 antibody (ET7106-63) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-63) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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