HIF-1 beta Recombinant Rabbit Monoclonal Antibody [ST05-25]
Catalog# ET1609-67
HIF-1 beta Recombinant Rabbit Monoclonal Antibody [ST05-25]
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA750187
不含抗保成分
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unconjugated
Overview
Product Name
HIF-1 beta Recombinant Rabbit Monoclonal Antibody [ST05-25]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human HIF-1 beta aa 479-789 / 789.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 87 kDa
Positive Control
A549 cell lysate, Jurkat cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human liver tissue, human fallopian tube tissue, human placenta tissue, human uterus tissue.
Conjugation
unconjugated
Clone Number
ST05-25
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IHC-P
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1:50-1:200
Target
Function
AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. Arnt 2 (aryl hydrocarbon receptor nuclear translocator 2), also known as Hif-2b or bHLHe1, is a 712 amino acid nuclear protein that is exclusively expressed in adult brain and kidney. Containing a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains, Arnt 2 specifically recognizes the xenobiotic response element (XRE).
Background References
1. Bian Y., et al. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics 96:253-262(2014).
2. Van Damme P., et al. N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
Subcellular Location
Nucleus.
Synonyms
arnT antibody
ARNT protein antibody
ARNT_HUMAN antibody
Aryl hydrocarbon receptor nuclear translocator antibody
bHLHe2 antibody
Class E basic helix-loop-helix protein 2 antibody
Dioxin receptor antibody
Dioxin receptor nuclear translocator antibody
Drnt antibody
HIF 1 beta antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of HIF-1 beta on different lysates with Rabbit anti-HIF-1 beta antibody (ET1609-67) at 1/5,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si HIF-1 beta cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 72/87 kDa
Exposure time: 1 minute 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-67) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of HIF-1 beta on different lysates with Rabbit anti-HIF-1 beta antibody (ET1609-67) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (10 µg/Lane)
Lane 2: Mouse testis tissue lysate (20 µg/Lane)
Lane 3: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 87 kDa
Observed band size: 72/87 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-67) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HIF-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using anti-HIF-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HIF-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-HIF-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-67, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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