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Gephyrin Recombinant Rabbit Monoclonal Antibody [JG34-47]

Usd: 385

Specification

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Catalog# ET7108-04

Gephyrin Recombinant Rabbit Monoclonal Antibody [JG34-47]

Application

  • WB

  • IHC-P

  • VIVIT

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Gephyrin Recombinant Rabbit Monoclonal Antibody [JG34-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Gephyrin aa 5-95 / 736.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, VIVIT

Molecular Weight

Predicted band size: 80 kDa

Positive Control

SK-Br-3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, human kidney tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

JG34-47

RRID

AB_3070807

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:400

Target

Function

The sub-membraneous region at the postsynaptic membrane contains a number of proteins critical for receptor targeting. Gephyrin is a microtubule-associated protein highly expressed in brain and localized to neuronal postsynaptic membranes. Gephyrin is essential for the postsynaptic localization of the inhibitory glycine receptor and is thought to anchor the receptor to subsynaptic microtubules. The protein is expressed in most mammalian tissues with predominant expression in brain. At least five additional splice variants of Gephyrin ranging in molecular weight have been identified in rat and human brain tissue.

Background References

1. Dejanovic B et al. Simultaneous impairment of neuronal and metabolic function of mutated gephyrin in a patient with epileptic encephalopathy. EMBO Mol Med 7:1580-1594 (2015).

2. Reiss J et al. A GPHN point mutation leading to molybdenum cofactor deficiency. Clin Genet 80:598-599 (2011).

Sequence Similarity

In the N-terminal section; belongs to the MoaB/Mog family.; In the C-terminal section; belongs to the MoeA family.

Subcellular Location

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse.

UNIPROT

SwissProt: Q9NQX3 Human

SwissProt: Q8BUV3 Mouse

SwissProt: Q03555 Rat

Synonyms

Domain E antibody

Domain G antibody

GEPH antibody

GEPH_HUMAN antibody

GPH antibody

GPHN antibody

GPHRYN antibody

KIAA1385 antibody

Molybdopterin molybdenumtransferase antibody

MPT adenylyltransferase antibody

Expand

Images

  • Western blot analysis of Gephyrin on different lysates with Rabbit anti-Gephyrin antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/1,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate (15 µg/Lane)<br />Lane 2: Neuro-2a cell lysate (15 µg/Lane)<br />Lane 3: C6 cell lysate (15 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (30 µg/Lane)<br />Lane 6: Mouse kidney tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 80 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Gephyrin on different lysates with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/1,000 dilution.

    Lane 1: SK-Br-3 cell lysate (15 µg/Lane)
    Lane 2: Neuro-2a cell lysate (15 µg/Lane)
    Lane 3: C6 cell lysate (15 µg/Lane)
    Lane 4: Mouse brain tissue lysate (30 µg/Lane)
    Lane 5: Mouse liver tissue lysate (30 µg/Lane)
    Lane 6: Mouse kidney tissue lysate (30 µg/Lane)

    Predicted band size: 80 kDa
    Observed band size: 80 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Gephyrin antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Gephyrin antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation