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Factor H Recombinant Rabbit Monoclonal Antibody [JA52-13]

Usd: 385

Specification

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Catalog# ET1704-83

Factor H Recombinant Rabbit Monoclonal Antibody [JA52-13]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Factor H Recombinant Rabbit Monoclonal Antibody [JA52-13]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Factor H aa 131-180 / 1231.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

140 kDa

Positive Control

Human kidney tissue lysates, Hela, HepG2, human liver tissue.

Conjugation

unconjugated

Clone Number

JA52-13

RRID

AB_3070526

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:100

  • IHC-P

  • 1:50-1:100

Target

Function

The Factor H gene family is a multidomain, multifunctional protein family whose individual members are defined by conserved structural elements, which display diverse yet often overlapping functions. These proteins share a common structural motif, the short consensus repeat (SCR), which is structurally conserved among related genes and between phylogenetically divergent species. The human complement Factor H (FH, CFH, HUS, b-1H) gene encodes a 1213 amino acid serum glycoprotein which is arranged into 20 SCRs, each approximately 60 amino acids long, and an 18-residue leader sequence. Factor H controls the function of the alternative complement pathway and acts as a cofactor with Factor I (C3b inactivator). In addition, Factor H has functional activity outside of the complement system, where it can bind to the cellular integrin receptor (CD11b/CD18), interact with cell surface glycosaminoglycans and associate with the surface of certain pathogenic microorganisms. Deficiencies in Factor H is a common characteristic of acute renal disease.

Background References

1. Ermert D et al. Virulence of Group A Streptococci Is Enhanced by Human Complement Inhibitors. PLoS Pathog 11:e1005043 (2015).

2. Segers FM et al. Complement alternative pathway activation in human nonalcoholic steatohepatitis. PLoS One 9:e110053 (2014).

Tissue Specificity

Expressed in the retinal pigment epithelium (at protein level). CFH is one of the most abundant complement components in blood where the liver is the major source of CFH protein in vivo. in addition, CFH is secreted by additional cell types including monocytes, fibroblasts, or endothelial cells.

Post-translational Modification

Sulfated on tyrosine residues.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P08603 Human

Synonyms

adrenomedullin binding protein antibody

age related maculopathy susceptibility 1 antibody

AHUS 1 antibody

AHUS1 antibody

AMBP 1 antibody

AMBP1 antibody

ARMD 4 antibody

ARMD4 antibody

ARMS 1 antibody

ARMS1 antibody

Expand

Images

  • Western blot analysis of Factor H on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-83" style="font-weight: bold;text-decoration: underline;">ET1704-83</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Factor H on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Factor H in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1704-83" style="font-weight: bold;text-decoration: underline;">ET1704-83</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Factor H in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Factor H in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1704-83" style="font-weight: bold;text-decoration: underline;">ET1704-83</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Factor H in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Factor H antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-83" style="font-weight: bold;text-decoration: underline;">ET1704-83</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Factor H antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-83, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation