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FGF2 Recombinant Rabbit Monoclonal Antibody [JM28-10]

Usd: 385

Specification

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Catalog# ET1703-18

FGF2 Recombinant Rabbit Monoclonal Antibody [JM28-10]

Application

  • WB

  • IP

  • FC

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FGF2 Recombinant Rabbit Monoclonal Antibody [JM28-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human FGF2 aa 121-170 / 288.

Species Reactivity

Human, Mouse

Validated Applications

WB, IP, FC, IHC-P

Molecular Weight

Predicted band size: 31 kDa

Positive Control

K-562 cell lysate, U-87 MG cell lysate, HeLa cell lysate, 293T cell lysate, human breast tissue, human prostate tissue, mouse trachea tissue, Jurkat.

Conjugation

unconjugated

Clone Number

JM28-10

RRID

AB_3070375

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IP

  • 1-2μg/sample

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:200-1:1,000

Target

Function

FGF2, also known as basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins, themselves a family of closely related molecules. Fibroblast growth factor protein was first purified in 1975; soon thereafter three variants were isolated: 'basic FGF' (FGF2); Heparin-binding growth factor-2; and Endothelial cell growth factor-2. Gene sequencing revealed that this group is the same FGF2 protein and is a member of a family of FGF proteins. Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.

Background References

1. Park IS et al. Enhancement of Ischemic Wound Healing by Spheroid Grafting of Human Adipose-Derived Stem Cells Treated with Low-Level Light Irradiation. PLoS One 10:e0122776 (2015).

2. Rotschafer JH et al. Modulation of neural stem/progenitor cell proliferation during experimental Herpes Simplex encephalitis is mediated by differential FGF-2 expression in the adult brain. Neurobiol Dis 58:144-55 (2013).

Sequence Similarity

Belongs to the heparin-binding growth factors family.

Tissue Specificity

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

Post-translational Modification

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.; Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

Subcellular Location

Secreted, Nucleus.

UNIPROT

SwissProt: P09038 Human

SwissProt: P15655 Mouse

Synonyms

Basic fibroblast growth factor antibody

Basic fibroblast growth factor bFGF antibody

BFGF antibody

FGF 2 antibody

FGF B antibody

FGF-2 antibody

Fgf2 antibody

FGF2 basic antibody

FGF2_HUMAN antibody

FGFB antibody

Expand

Images

  • Western blot analysis of FGF2 on different lysates with Rabbit anti-FGF2 antibody (<a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: 293T cell lysate (20 µg/Lane)<br /><br />Predicted band size: 31 kDa<br />Observed band size: 18/22/24 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FGF2 on different lysates with Rabbit anti-FGF2 antibody (ET1703-18) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: 293T cell lysate (20 µg/Lane)

    Predicted band size: 31 kDa
    Observed band size: 18/22/24 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-18) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-FGF2 antibody (<a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-FGF2 antibody (ET1703-18) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-18) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-FGF2 antibody (<a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-FGF2 antibody (ET1703-18) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-18) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse trachea tissue with Rabbit anti-FGF2 antibody (<a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse trachea tissue with Rabbit anti-FGF2 antibody (ET1703-18) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-18) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FGF2 was immunoprecipitated from 0.2 mg K-562 cell lysate with <a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: K-562 cell lysate (input)<br />Lane 2: <a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a> IP in K-562 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1703-18" style="font-weight: bold;text-decoration: underline;">ET1703-18</a> in K-562 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute; ECL: K1802

    FGF2 was immunoprecipitated from 0.2 mg K-562 cell lysate with ET1703-18 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-18 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: K-562 cell lysate (input)
    Lane 2: ET1703-18 IP in K-562 cell lysate
    Lane 3: Rabbit IgG instead of ET1703-18 in K-562 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute; ECL: K1802

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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