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EGR2 Recombinant Rabbit Monoclonal Antibody [JG78-39]

Usd: 385

Specification

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Catalog# ET7108-57

EGR2 Recombinant Rabbit Monoclonal Antibody [JG78-39]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

EGR2 Recombinant Rabbit Monoclonal Antibody [JG78-39]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human EGR2 aa 2-96 / 476.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 50 kDa

Positive Control

SH-SY5Y cell lysate, HepG2 cell lysate, Daudi cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

JG78-39

RRID

AB_3070855

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

Target

Function

The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Binds to two specific DNA sites located in the promoter region of HOXA4. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene.

Background References

1. Garcia-Gutierrez P. et al. The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators. EMBO Rep. 12:1018-1023(2011).

2. Funalot B. et al. Homozygous deletion of an EGR2 enhancer in congenital amyelinating neuropathy. Ann. Neurol. 71:719-723(2012).

Sequence Similarity

Belongs to the EGR C2H2-type zinc-finger protein family.

Post-translational Modification

Ubiquitinated by WWP2 leading to proteasomal degradation.; Acetylated at Lys-247. May be deacetylated by HDAC6, HDAC10 or SIRT1.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P11161 Human

Synonyms

AT591 antibody

CMT1D antibody

CMT4E antibody

DKFZp686J1957 antibody

E3 SUMO-protein ligase EGR2 antibody

Early growth response 2 antibody

Early growth response protein 2 antibody

EGR-2 antibody

egr2 antibody

EGR2_HUMAN antibody

Expand

Images

  • Western blot analysis of EGR2 on different lysates with Rabbit anti-EGR2 antibody (<a href="/products/ET7108-57" style="font-weight: bold;text-decoration: underline;">ET7108-57</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: Daudi cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-57" style="font-weight: bold;text-decoration: underline;">ET7108-57</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EGR2 on different lysates with Rabbit anti-EGR2 antibody (ET7108-57) at 1/1,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Daudi cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-57) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation