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DOPA Decarboxylase Recombinant Rabbit Monoclonal Antibody [JA53-16]

Usd: 385

Specification

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Catalog# ET1704-94

DOPA Decarboxylase Recombinant Rabbit Monoclonal Antibody [JA53-16]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

DOPA Decarboxylase Recombinant Rabbit Monoclonal Antibody [JA53-16]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human DOPA Decarboxylase aa 27-76 / 480.

Species Reactivity

Human, Mouse (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 54 kDa

Positive Control

Human kidney tissue, human liver tissue, mouse liver tissue lysates, HepG2 cell lysates.

Conjugation

unconjugated

Clone Number

JA53-16

RRID

AB_3070536

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:400

Target

Function

DOPA decarboxylase (DDC), also designated aromatic-L-amino-acid decarboxylase (AADC) belongs to the group II decarboxylase family of proteins. DDC, which can form a homodimer, is an important protein in the catecholamine biosynthesis pathway. DDC acts as a catalyst in the decarboxylation of L-5-hydroxytryptophan to serotonin, L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-tryptophan to tryptamine. Defects in the gene encoding for DDC may cause the autosomal recessive disorder AADC deficiency. AADC deficiency is an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency. This causes poor feeding, psychomotor and developmental delays, lethargy, ptosis, gastrointestinal disturbances and hypothermia.

Background References

1. Wienecke J et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014).

2. Panayotis N et al. Morphological and functional alterations in the substantia nigra pars compacta of the Mecp2-null mouse. Neurobiol Dis 41:385-97 (2011).

Sequence Similarity

Belongs to the group II decarboxylase family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P20711 Human

SwissProt: O88533 Mouse

SwissProt: P14173 Rat

Synonyms

AADC antibody

Aromatic L Amino Acid Decarboxylase antibody

Aromatic-L-amino-acid decarboxylase antibody

Ddc antibody

DDC_HUMAN antibody

DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody

DOPA decarboxylase antibody

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DOPA Decarboxylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-94" style="font-weight: bold;text-decoration: underline;">ET1704-94</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DOPA Decarboxylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DOPA Decarboxylase antibody (<a href="/products/ET1704-94" style="font-weight: bold;text-decoration: underline;">ET1704-94</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-94" style="font-weight: bold;text-decoration: underline;">ET1704-94</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DOPA Decarboxylase antibody (ET1704-94) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-94) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of DOPA Decarboxylase on mouse liver tissue lysates with Rabbit anti-DOPA Decarboxylase antibody (<a href="/products/ET1704-94" style="font-weight: bold;text-decoration: underline;">ET1704-94</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-94" style="font-weight: bold;text-decoration: underline;">ET1704-94</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DOPA Decarboxylase on mouse liver tissue lysates with Rabbit anti-DOPA Decarboxylase antibody (ET1704-94) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-94) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of DOPA Decarboxylase on HepG2 cell lysates with Rabbit anti-DOPA Decarboxylase antibody (<a href="/products/ET1704-94" style="font-weight: bold;text-decoration: underline;">ET1704-94</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-94" style="font-weight: bold;text-decoration: underline;">ET1704-94</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DOPA Decarboxylase on HepG2 cell lysates with Rabbit anti-DOPA Decarboxylase antibody (ET1704-94) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 55 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-94) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation