DIAPH1 Recombinant Rabbit Monoclonal Antibody [JE40-59]
Catalog# ET7109-11
DIAPH1 Recombinant Rabbit Monoclonal Antibody [JE40-59]
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
DIAPH1 Recombinant Rabbit Monoclonal Antibody [JE40-59]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human DIAPH1 aa 1-50 / 1,272.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P
Molecular Weight
141 kDa
Positive Control
SiHa, LOVO, human liver cancer tissue, human colon tissue.
Conjugation
unconjugated
Clone Number
JE40-59
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000-1:10,000
-
IF-Cell
-
1:50
-
IHC-P
-
1:50-1:100
Target
Function
Dia 1, also known as DIAPH1 (diaphanous homolog 1) or DRF1, a mammalian homolog of the Drosophila diaphanous gene, belongs to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 (formin homology 1) and FH2 (formin homology 2) domains in their carboxy terminal regions. Dia 1 contains a DAD (diaphanous autoregulatory) domain, which is involved in the elongation of actin filaments, and a GBD/FH3 (Rho GTPase-binding/formin homology 3) domain, which interacts with the DAD domain via autoinhibitory interactions to regulate the activation of Dia 1. Dia 1 is required for the assembly of F-actin structures, and regulates the polymerization and depolymerization of actin filaments. Localizing to the cell membrane, Dia 1 is expressed in a wide range of tissues, including brain, heart, lung and kidney. Defects to the gene encoding Dia 1 have been linked to deafness autosomal dominant type 1 (DFNA1), a disorder characterized by sensorineural hearing loss..
Background References
1. Zaoui K et al. ErbB2 receptor controls microtubule capture by recruiting ACF7 to the plasma membrane of migrating cells. Proc Natl Acad Sci USA 107:18517-18522 (2010).
2. Bai S W et al. Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration. BMC Biol 9:54-54 (2011).
Sequence Similarity
Belongs to the formin homology family. Diaphanous subfamily.
Tissue Specificity
Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets.
Post-translational Modification
Phosphorylation at Thr-768 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement.
Subcellular Location
Cytoskeleton. Plasma membrane.
UNIPROT
Synonyms
DIAPH1 antibody
deafness, autosomal dominant 1 antibody
DFNA1 antibody
DIA1 antibody
DIAP1 antibody
DIAP1_HUMAN antibody
DIAPH1 antibody
Diaphanous homolog 1 (Drosophila) antibody
diaphanous homolog 1 antibody
Diaphanous related formin 1 antibody
ExpandImages
-
Western blot analysis of DIAPH1 on SiHa cell lysate using anti-DIAPH1 antibody at 1/5,000 dilution.
-
ICC staining DIAPH1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"