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Cyclin A1+ Cyclin A2 Recombinant Rabbit Monoclonal Antibody [SD2053]

Usd: 385

Specification

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Catalog# ET1612-8

Cyclin A1+ Cyclin A2 Recombinant Rabbit Monoclonal Antibody [SD2053]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cyclin A1+ Cyclin A2 Recombinant Rabbit Monoclonal Antibody [SD2053]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Cyclin A1 aa 253-440 / 465.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Molecular Weight

52/49 kDa

Positive Control

Hela cell lysate, HepG2 cell lysate, human colon carcinoma tissue.

Conjugation

unconjugated

Clone Number

SD2053

RRID

AB_3070156

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. Cyclin A2 is a member of the highly conserved cyclin family. Cyclins regulate CDK kinases and different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin A2 is expressed in all tissues tested, in contrast to cyclin A1, which is present only in germ cells. The Cyclin A2 protein binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 also functions in the transition to DNA replication and synthesis phases of the cell cycle and is quickly destroyed as the cell moves into mitosis.

Background References

1. Pakula A et al. Altered expression of cyclin A 1 in muscle of patients with facioscapulohumeral muscle dystrophy (FSHD-1). PLoS One 8:e73573 (2013).

2. Sharon E et al. Human herpesvirus 6 (HHV-6) alters E2F1/Rb pathways and utilizes the E2F1 transcription factor to express viral genes. Proc Natl Acad Sci U S A 111:451-6 (2014).

Sequence Similarity

Belongs to the cyclin family. Cyclin AB subfamily.

Post-translational Modification

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: P20248 Human

SwissProt: P78396 Human

Synonyms

CCN1 antibody

CCNA antibody

CCNA1 antibody

CCNA2 antibody

CT146 antibody

Cyclin-A antibody

Cyclin-A1 antibody

Cyclin-A2 antibody

Images

  • Western blot analysis of Cyclin A1+ Cyclin A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-8" style="font-weight: bold;text-decoration: underline;">ET1612-8</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Hela cell lysate<br />Lane 2: HepG2 cell lysate

    Western blot analysis of Cyclin A1+ Cyclin A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Cyclin A1+ Cyclin A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-8" style="font-weight: bold;text-decoration: underline;">ET1612-8</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Cyclin A1+ Cyclin A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation