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Caspase-6 p18 Recombinant Rabbit Monoclonal Antibody [SN07-26]

Usd: 385

Specification

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Catalog# ET1611-83

Caspase-6 p18 Recombinant Rabbit Monoclonal Antibody [SN07-26]

Application

  • WB

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Caspase-6 p18 Recombinant Rabbit Monoclonal Antibody [SN07-26]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Caspase-6 p18 aa 25-182 / 293.

Species Reactivity

Human

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 33 kDa

Positive Control

HCT 116 cell lysate, U-937 cell lysate.

Conjugation

unconjugated

Clone Number

SN07-26

RRID

AB_3070063

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IP

  • 1-2μg/sample

Target

Function

Caspase-6 is an enzyme that in humans is encoded by the CASP6 gene.CASP6 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-6 has known functions in apoptosis,early immune response and neurodegeneration in Huntington's and Alzheimer's disease.

Background References

1. Siendones, E. et al. 2005. PGE1 abolishes the mitochondrial-independent cell death pathway induced by D-galactosamine in primary culture of rat hepatocytes J Gastroenterol Hepatol.. 20: 108-116.

2. Yang, X.H. et al. 2005. Reconstitution of caspase-3 sensitizes MCF-7 breast cancer cells to radiation therapy Int J Oncol.. 26: 1675-1680.

Sequence Similarity

Belongs to the peptidase C14A family.

Post-translational Modification

Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P55212 Human

Synonyms

Caspase6

Apoptotic protease Mch-2 antibody

Apoptotic protease MCH2 antibody

CASP-6 antibody

CASP6 antibody

CASP6_HUMAN antibody

Caspase 6 antibody

Caspase 6 apoptosis related cysteine protease antibody

Caspase 6, apoptosis related cysteine peptidase antibody

Caspase-6 subunit p11 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Caspase-6 on different lysates with Rabbit anti-Caspase-6 antibody (<a href="/products/ET1611-83" style="font-weight: bold;text-decoration: underline;">ET1611-83</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si Caspase-6 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 8 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-83" style="font-weight: bold;text-decoration: underline;">ET1611-83</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Caspase-6 on different lysates with Rabbit anti-Caspase-6 antibody (ET1611-83) at 1/1,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si Caspase-6 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 35 kDa

    Exposure time: 8 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-83) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Caspase-6 on different lysates with Rabbit anti-Caspase-6 antibody (<a href="/products/ET1611-83" style="font-weight: bold;text-decoration: underline;">ET1611-83</a>) at 1/2,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate<br />Lane 2: U-937 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-83" style="font-weight: bold;text-decoration: underline;">ET1611-83</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Caspase-6 on different lysates with Rabbit anti-Caspase-6 antibody (ET1611-83) at 1/2,000 dilution.

    Lane 1: HCT 116 cell lysate
    Lane 2: U-937 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 35 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-83) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"