CHD1L Recombinant Rabbit Monoclonal Antibody [JG35-44]
Catalog# ET7108-14
CHD1L Recombinant Rabbit Monoclonal Antibody [JG35-44]
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WB
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IHC-P
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
CHD1L Recombinant Rabbit Monoclonal Antibody [JG35-44]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CHD1L aa 680-820 / 897.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 101 kDa
Positive Control
HepG2 cell lysate , human tonsil tissue, human colon cancer tissue, mouse testis tissue, HepG2.
Conjugation
unconjugated
Clone Number
JG35-44
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
Target
Function
CHD1L (Chromodomain helicase DNA-binding protein 1-like) is a 897 amino acid protein encoded by the human gene CHD1L. The CHD family of proteins are ATP-dependent chromatin remodeling enzymes, which combine chromodomains, with SWI2/SNF2 ATPase/helicase motifs and DNA-binding capability. Chromodomains are protein regions of about 40-50 amino acid residues found in proteins associated with chromatin remodeling and manipulation. The domain is highly conserved among both plants and animals and is found in a large variety of proteins from many genomes. CHD1L and CHD1 share most homology within two internal helicase domains.
Background References
1. Ma N-F et al. Isolation and characterization of a novel oncogene, amplified in liver cancer 1, within a commonly amplified region at 1q21 in hepatocellular carcinoma. Hepatology 47:503-510 (2008).
2. Ahel D et al. Poly(ADP-ribose)-dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1. Science 325:1240-1243 (2009).
Sequence Similarity
Belongs to the SNF2/RAD54 helicase family.
Tissue Specificity
Frequently overexpressed in hepatomacellular carcinomas.
Subcellular Location
Nucleus.
Synonyms
ALC1 antibody
Amplified in liver cancer 1 antibody
Amplified in liver cancer protein 1 antibody
chd1l antibody
CHD1L_HUMAN antibody
CHDL antibody
Chromodomain helicase DNA binding protein 1 like antibody
Chromodomain-helicase-DNA-binding protein 1-like antibody
FLJ22530 antibody
Images
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Western blot analysis of CHD1L on HepG2 cell lysate using anti-CHD1L antibody at 1/1,000 dilution.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"