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CENPC Recombinant Rabbit Monoclonal Antibody [JG36-15]

Usd: 385

Specification

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Catalog# ET7108-24

CENPC Recombinant Rabbit Monoclonal Antibody [JG36-15]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CENPC Recombinant Rabbit Monoclonal Antibody [JG36-15]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human CENPC aa 360-540 / 943.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 107 kDa

Positive Control

Daudi cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, mouse spleen tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

JG36-15

RRID

AB_3070824

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50

Target

Function

A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The evolutionarily conserved Centromere Protein-C, CENP-C, is a kinetochore assembly protein. CENP-C is located on the fibers of the kinetochore and constitutes a kinetochore organizing center that tightly associates with DNA. CENP-C is necessary for the formation of a functional centromere, which indicates that CENP-C is important for mitotic progression. In addition, CENP-C is lost from centromeres during herpes simplex virus 1 infection, causing substantial structural changes in the kinetochore, which suggests that the structure of CENP-C is regulated during the cell cycle.

Background References

1. Gopalakrishnan S et al. DNMT3B interacts with constitutive centromere protein CENP-C to modulate DNA methylation and the histone code at centromeric regions. Hum Mol Genet 18:3178-3193 (2009).

2. Gascoigne K E et al. Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes. Cell 145:410-422 (2011).

Sequence Similarity

Belongs to the CENP-C/MIF2 family.

Subcellular Location

Nucleus, kinetochore, centromere.

UNIPROT

SwissProt: Q03188 Human

SwissProt: P49452 Mouse

Entrez Gene: 305270 Rat

Synonyms

CENP-C 1 antibody

CENP-C antibody

CENPC_HUMAN antibody

CENPC1 antibody

Centromere autoantigen C antibody

Centromere autoantigen C1 antibody

Centromere protein C 1 antibody

Centromere protein C antibody

hcp-4 antibody

ICEN7 antibody

Expand

Images

  • Western blot analysis of CENPC on different lysates with Rabbit anti-CENPC antibody (<a href="/products/ET7108-24" style="font-weight: bold;text-decoration: underline;">ET7108-24</a>) at 1/1,000 dilution.<br /><br />Lane 1: Daudi cell lysate (20 µg/Lane)<br />Lane 2: HL-60 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: C2C12 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 107 kDa<br />Observed band size: 107 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-24" style="font-weight: bold;text-decoration: underline;">ET7108-24</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CENPC on different lysates with Rabbit anti-CENPC antibody (ET7108-24) at 1/1,000 dilution.

    Lane 1: Daudi cell lysate (20 µg/Lane)
    Lane 2: HL-60 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: C2C12 cell lysate (20 µg/Lane)

    Predicted band size: 107 kDa
    Observed band size: 107 kDa

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CENPC antibody (<a href="/products/ET7108-24" style="font-weight: bold;text-decoration: underline;">ET7108-24</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-24" style="font-weight: bold;text-decoration: underline;">ET7108-24</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CENPC antibody (ET7108-24) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CENPC antibody (<a href="/products/ET7108-24" style="font-weight: bold;text-decoration: underline;">ET7108-24</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-24" style="font-weight: bold;text-decoration: underline;">ET7108-24</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CENPC antibody (ET7108-24) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"