Skip to content

CEBP alpha Recombinant Rabbit Monoclonal Antibody [SD202-09]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1612-46

CEBP alpha Recombinant Rabbit Monoclonal Antibody [SD202-09]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CEBP alpha Recombinant Rabbit Monoclonal Antibody [SD202-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal Human CEBP alpha aa 309-358 / 358.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 38 kDa

Positive Control

THP-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

SD202-09

RRID

AB_3070120

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

The transcription factor C/EBP α (CCAAT-enhancer binding protein) is a heat-stable, sequence-specific DNA-binding protein that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growth-arrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including prolactin gene expression.

Background References

1. Christou-Kent M et al. CEBPA phase separation links transcriptional activity and 3D chromatin hubs. Cell Rep. 2023 Aug

2. Georgi JA et al. Prognostic impact of CEBPA mutational subgroups in adult AML. Leukemia. 2024 Feb

Sequence Similarity

Belongs to the bZIP family. C/EBP subfamily.

Post-translational Modification

Phosphorylation at Ser-190 is required for interaction with CDK2, CDK4 and SWI/SNF complex leading to cell cycle inhibition. Dephosphorylated at Ser-190 by protein phosphatase 2A (PP2A) through PI3K/AKT signaling pathway regulation. Phosphorylation at Thr-226 and Thr-230 by GSK3 is constitutive in adipose tissue and lung. In liver, both Thr-226 and Thr-230 are phosphorylated only during feeding but not during fasting. Phosphorylation of the GSK3 consensus sites selectively decreases transactivation activity on IRE-controlled promoters.; Sumoylated, sumoylation blocks the inhibitory effect on cell proliferation by disrupting the interaction with SMARCA2.; Ubiquitinated by COP1 upon interaction with TRIB1.

Subcellular Location

Nucleus, nucleolus.

UNIPROT

SwissProt: P49715 Human

SwissProt: P53566 Mouse

SwissProt: P05554 Rat

Synonyms

Apoptotic cysteine protease antibody

Apoptotic protease Mch 5 antibody

C/EBP alpha antibody

C/ebpalpha antibody

CAP4 antibody

Caspase 8 precursor antibody

CBF-A antibody

CCAAT Enhancer Binding Protein alpha antibody

CCAAT/enhancer binding protein (C/EBP), alpha antibody

CCAAT/enhancer-binding protein alpha antibody

Expand

Images

  • Western blot analysis of CEBP alpha on different lysates with Rabbit anti-CEBP alpha antibody (<a href="/products/ET1612-46" style="font-weight: bold;text-decoration: underline;">ET1612-46</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate (10 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (30 µg/Lane)<br />Lane 3: Rat liver tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 30/42 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-46" style="font-weight: bold;text-decoration: underline;">ET1612-46</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CEBP alpha on different lysates with Rabbit anti-CEBP alpha antibody (ET1612-46) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate (10 µg/Lane)
    Lane 2: Mouse liver tissue lysate (30 µg/Lane)
    Lane 3: Rat liver tissue lysate (30 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 30/42 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation