CD62P Recombinant Rabbit Monoclonal Antibody [JM10-95]
Catalog# ET1703-49
CD62P Recombinant Rabbit Monoclonal Antibody [JM10-95]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
CD62P Recombinant Rabbit Monoclonal Antibody [JM10-95]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CD62P aa 796-830/830.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 91 kDa
Positive Control
HUVEC cell lysate, human placenta tissue lysate, human tonsil tissue, human spleen tissue.
Conjugation
unconjugated
Clone Number
JM10-95
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:2,000
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IHC-P
-
1:50-1:200
Target
Function
Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes.
Background References
1. Wu F et al. CXCR2 is essential for cerebral endothelial activation and leukocyte recruitment during neuroinflammation. J Neuroinflammation 12:98 (2015).
2. K nnecke M et al. Immune imbalance in nasal polyps of caucasian chronic rhinosinusitis patients is associated with a downregulation of e-selectin. J Immunol Res 2014:959854 (2014).
Sequence Similarity
Belongs to the selectin/LECAM family.
Tissue Specificity
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Antigen CD62 antibody
CD62 antibody
CD62 antigen-like family member P antibody
CD62P antibody
GMP 140 antibody
GMP-140 antibody
GMP140 antibody
GMRP antibody
Granule membrane protein 140 antibody
Granulocyte membrane protein antibody
ExpandImages
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Western blot analysis of CD62P on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HUVEC cell lysate
Lane 2: Human placenta tissue lysate
Predicted band size: 91 kDa
Observed band size: 100 kDa -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"