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CD16 Recombinant Rabbit Monoclonal Antibody [JE49-79]

Usd: 385

Specification

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Catalog# ET7109-97

CD16 Recombinant Rabbit Monoclonal Antibody [JE49-79]

Application

  • WB

Reactivity

  • Mouse

  • Rat

Predicted reactivity

  • Human

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD16 Recombinant Rabbit Monoclonal Antibody [JE49-79]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human CD16 aa 151-254 / 254.

Species Reactivity

Mouse, Rat (Predicted: Human)

Predicted species support after-sales service

Validated Applications

WB

Molecular Weight

Predicted band size: 29 kDa

Positive Control

RAW264.7 cell lysate, Rat spleen tissue lysate, Rat thymus tissue lysate.

Conjugation

unconjugated

Clone Number

JE49-79

RRID

AB_3070969

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, and macrophages. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16. CD16 is the type III Fcγ receptor. In humans, it exists in two different forms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which have 96% sequence similarity in the extracellular immunoglobulin binding regions. While FcγRIIIa is expressed on mast cells, macrophages, and natural killer cells as a transmembrane receptor, FcγRIIIb is only expressed on neutrophils. In addition, FcγRIIIb is the only Fc receptor anchored to the cell membrane by a glycosyl-phosphatidylinositol (GPI) linker, and also plays a significant role in triggering calcium mobilization and neutrophil degranulation. FcγRIIIa and FcγRIIIb together are able to activate degranulation, phagocytosis, and oxidative burst, which allows neutrophils to clear opsonized pathogens.

Background References

1. Mizushima T.et.al.Structural basis for improved efficacy of therapeutic antibodies on defucosylation of their Fc glycans.Genes Cells 16:1071-1080(2011).

Tissue Specificity

Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.

Post-translational Modification

Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.; The soluble form is produced by a proteolytic cleavage.

Subcellular Location

Cell membrane, Membrane, Secreted.

UNIPROT

SwissProt: P08637 Human

SwissProt: P08508 Mouse

SwissProt: P27645 Rat

Synonyms

CD 16 antibody

CD 16a antibody

CD16 antibody

CD16a antibody

CD16a antigen antibody

CD16B antibody

CD16b antigen antibody

Fc fragment of IgG antibody

Fc fragment of IgG low affinity IIIa receptor (CD16) antibody

Fc fragment of IgG low affinity IIIa receptor antibody

Expand

Images

  • Western blot analysis of CD16 on different lysates with Rabbit anti-CD16 antibody (<a href="/products/ET7109-97" style="font-weight: bold;text-decoration: underline;">ET7109-97</a>) at 1/1,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 2: Rat spleen tissue lysate (40 µg/Lane)<br />Lane 3: Rat thymus tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 29 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7109-97" style="font-weight: bold;text-decoration: underline;">ET7109-97</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD16 on different lysates with Rabbit anti-CD16 antibody (ET7109-97) at 1/1,000 dilution.

    Lane 1: RAW264.7 cell lysate (20 µg/Lane)
    Lane 2: Rat spleen tissue lysate (40 µg/Lane)
    Lane 3: Rat thymus tissue lysate (40 µg/Lane)

    Predicted band size: 29 kDa
    Observed band size: 40 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-97) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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