Bcl-6 Recombinant Rabbit Monoclonal Antibody [JB18-48]
Catalog# ET7107-80
Bcl-6 Recombinant Rabbit Monoclonal Antibody [JB18-48]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
Bcl-6 Recombinant Rabbit Monoclonal Antibody [JB18-48]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Bcl-6 aa 657-706 / 706.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 79 kDa
Positive Control
Ramos cell lysate, Raji cell lysate, Daudi cell lysate.
Conjugation
unconjugated
Clone Number
JB18-48
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
Target
Function
Bcl-6, a transcriptional repressor, binds Stat recognition-like DNA elements and influences germinal center development and Th1/Th2 differentiation. Bcl-6 negatively regulates NFkB expression, thereby inhibiting NFkB-mediated cellular functions. HDAC- and silent information regulator (SIR)-2-dependent acet-ylation of Bcl-6 causes downregulation of activity by inhibiting the ability of Bcl-6 to recruit complexes containing histone deacetylases (HDACs). Bcl-6 is frequently deregulated in non-Hodgkin's B cell lymphomas. The human BCL6 gene has been shown to encode a protein of 706 amino acids.
Background References
1. Niu H et al. Antigen receptor signaling induces MAP kinase-mediated phosphorylation and degradation of the BCL-6 transcription factor. Genes Dev 12:1953-1961 (1998).
2. Shaffer A L et al. BCL-6 represses genes that function in lymphocyte differentiation, inflammation, and cell cycle control. Immunity 13:199-212 (2000).
Tissue Specificity
Expressed in germinal center T- and B-cells and in primary immature dendritic cells.
Post-translational Modification
Phosphorylated by MAPK1 in response to antigen receptor activation at Ser-333 and Ser-343. Phosphorylated by ATM in response to genotoxic stress. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.; Polyubiquitinated. Polyubiquitinated by SCF(FBXO11), leading to its degradation by the proteasome. Ubiquitinated by the SCF(FBXL17) complex, leading to its degradation by the proteaseome: ubiquitination by the SCF(FBXL17) complex takes place when aberrant BTB domain dimers are formed.; Acetylated at Lys-379 by EP300 which inhibits the interaction with NuRD complex and the transcriptional repressor function. Deacetylated by HDAC- and SIR2-dependent pathways.
Subcellular Location
Nucleus.
Synonyms
B Cell Lymphoma 6 Protein antibody
B-cell CLL/lymphoma 6 antibody
B-cell lymphoma 5 protein antibody
B-cell lymphoma 6 protein transcript antibody
BCL5 antibody
BCL6A antibody
cys-his2 zinc finger transcription factor antibody
LAZ3 antibody
lymphoma-associated zinc finger gene on chromosome 3 antibody
Protein LAZ-3 antibody
ExpandImages
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Western blot analysis of Bcl-6 on different lysates with Rabbit anti-Bcl-6 antibody (ET7107-80) at 1/2,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Raji cell lysate
Lane 3: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-80) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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