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Aryl hydrocarbon Receptor Recombinant Rabbit Monoclonal Antibody [JM34-10]

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Specification

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Catalog# ET1703-11

Aryl hydrocarbon Receptor Recombinant Rabbit Monoclonal Antibody [JM34-10]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Aryl hydrocarbon Receptor Recombinant Rabbit Monoclonal Antibody [JM34-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Aryl hydrocarbon Receptor aa 735-775 / 848.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 96 kDa

Positive Control

HepG2 cell lysate, A549 cell lysate.

Conjugation

unconjugated

Clone Number

JM34-10

RRID

AB_3070368

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

The aryl hydrocarbon receptor (also known as AhR, AHR, ahr, ahR, or dioxin receptor) is a protein that in humans is encoded by the AHR gene. The aryl hydrocarbon receptor is a transcription factor that regulates gene expression. It was originally thought to function primarily as a sensor of xenobiotic chemicals and also as the regulator of enzymes such as cytochrome P450s that metabolize these chemicals. The most notable of these xenobiotic chemicals are aromatic (aryl) hydrocarbons from which the receptor derives its name. In addition to regulating metabolism enzymes, the AhR has roles in regulating immunity, stem cell maintenance, and cellular differentiation. The aryl hydrocarbon receptor is a member of the family of basic helix-loop-helix transcription factors. AHR binds several exogenous ligands such as natural plant flavonoids, polyphenolics and indoles, as well as synthetic polycyclic aromatic hydrocarbons and dioxin-like compounds. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. Upon ligand binding to chemicals such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the chaperones dissociate resulting in AhR translocating into the nucleus and dimerizing with ARNT (AhR nuclear translocator), leading to changes in gene transcription.

Background References

1. Bunaciu RP et al. 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS One 10:e0135668 (2015).

2. Bessede A et al. Aryl hydrocarbon receptor control of a disease tolerance defence pathway. Nature 511:184-90 (2014).

Tissue Specificity

Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. Expressed in retinal photoreceptors.

Post-translational Modification

Mono-ADP-ribosylated, leading to inhibit transcription activator activity of AHR.

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P35869 Human

Synonyms

Ah receptor antibody

AhR antibody

AHR_HUMAN antibody

Aromatic hydrocarbon receptor antibody

Aryl hydrocarbon receptor antibody

Aryl hydrocarbon receptor precursor antibody

bHLHe76 antibody

Class E basic helix loop helix protein 76 antibody

Class E basic helix-loop-helix protein 76 antibody

HGNC:348 antibody

Images

  • Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody (<a href="/products/ET1703-11" style="font-weight: bold;text-decoration: underline;">ET1703-11</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: A549 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 96 kDa<br />Observed band size: 105 kDa<br /><br />Exposure time: 2 minutes 37 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-11" style="font-weight: bold;text-decoration: underline;">ET1703-11</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody (ET1703-11) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: A549 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 96 kDa
    Observed band size: 105 kDa

    Exposure time: 2 minutes 37 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody (<a href="/products/ET1703-11" style="font-weight: bold;text-decoration: underline;">ET1703-11</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si Aryl hydrocarbon Receptor cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 96 kDa<br />Observed band size: 105 kDa<br /><br />Exposure time: 2 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-11" style="font-weight: bold;text-decoration: underline;">ET1703-11</a>) at 1/1,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody (ET1703-11) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si Aryl hydrocarbon Receptor cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 96 kDa
    Observed band size: 105 kDa

    Exposure time: 2 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-11) at 1/1,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation