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Angiotensin II Type 2 Receptor Recombinant Rabbit Monoclonal Antibody [SN71-03]

Usd: 385

Specification

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Catalog# ET1611-41

Angiotensin II Type 2 Receptor Recombinant Rabbit Monoclonal Antibody [SN71-03]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Angiotensin II Type 2 Receptor Recombinant Rabbit Monoclonal Antibody [SN71-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Angiotensin II Type 2 Receptoraa 311-363 / 363.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 41 kDa

Positive Control

Mouse kidney tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, 293T cell lysate, Hela cell lysate.

Conjugation

unconjugated

Clone Number

SN71-03

RRID

AB_3070020

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

Angiotensin II (Ang II) is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. Although Ang II interacts with two types of cell surface receptors, AT1 and AT2, most of the major cardiovascular effects seem to be mediated through AT1. Molecular cloning of the AT1 protein has shown it to be a member of the G protein-associated seven transmembrane protein receptor family. Ang II treatment of cells results in activation of several signal transduction pathways as evidenced by tyrosine phosphorylation of several proteins and induction of others. PLCγ is phosphorylated after 30 seconds of treatment with Angiotensin II, indicating this as an early signal transduction event. Ang II treatment also stimulates phosphorylation of Shc, FAK and MAP kinases, and induces MKP-1, indicating stimulation of growth factor pathways. Ang II stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by coimmunoprecipitation. The AT1 receptor has no cytoplasmic kinase domain, but is able to function as a substrate for Src kinases and has several putative phosphorylation sites.

Background References

1. Wang J et al. Qishenyiqi Dropping Pill attenuates myocardial fibrosis in rats by inhibiting RAAS-mediated arachidonic acid inflammation. J Ethnopharmacol 176:375-84 (2015).

2. Goyal R et al. Antenatal maternal protein deprivation: sexually dimorphic programming of the pancreatic renin-angiotensin system. J Renin Angiotensin Aldosterone Syst 14:137-45 (2013).

Sequence Similarity

Belongs to the G-protein coupled receptor 1 family.

Tissue Specificity

In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Expressed in the cerebellum. Very highly expressed in fetal kidney and intestine.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P50052 Human

SwissProt: P35374 Mouse

SwissProt: P35351 Rat

Synonyms

AGTR 2 antibody

Agtr2 antibody

AGTR2_HUMAN antibody

angiotensin II receptor type 2 antibody

Angiotensin II type-2 receptor antibody

Angiotensin receptor 2 antibody

AT 2 antibody

AT2 antibody

ATGR 2 antibody

ATGR2 antibody

Expand

Images

  • Western blot analysis of Angiotensin II Type 2 Receptor on different lysates with Rabbit anti-Angiotensin II Type 2 Receptor antibody (<a href="/products/ET1611-41" style="font-weight: bold;text-decoration: underline;">ET1611-41</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse kidney tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Rat liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: Lane 1-2: 30 seconds; Lane 3: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-41" style="font-weight: bold;text-decoration: underline;">ET1611-41</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Angiotensin II Type 2 Receptor on different lysates with Rabbit anti-Angiotensin II Type 2 Receptor antibody (ET1611-41) at 1/1,000 dilution.

    Lane 1: Mouse kidney tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Rat liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 45 kDa

    Exposure time: Lane 1-2: 30 seconds; Lane 3: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Angiotensin II Type 2 Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-41" style="font-weight: bold;text-decoration: underline;">ET1611-41</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: 293T cell lysate<br />Lane 2: Hela cell lysate

    Western blot analysis of Angiotensin II Type 2 Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 293T cell lysate
    Lane 2: Hela cell lysate

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation