Alpha Fodrin Recombinant Rabbit Monoclonal Antibody [JU32-09]
Catalog# ET1706-44
Alpha Fodrin Recombinant Rabbit Monoclonal Antibody [JU32-09]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Alpha Fodrin Recombinant Rabbit Monoclonal Antibody [JU32-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Alpha Fodrin aa 46-95 / 2,472.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 285 kDa
Positive Control
Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, human liver carcinoma tissue, human pancreas tissue, mouse cerebellum tissue.
Conjugation
unconjugated
Clone Number
JU32-09
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
Alpha Fodrin also named Spectrin. Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous α and β chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Spectrin α I and spectrin β I are present in erythrocytes, whereas spectrin α II (also designated fodrin α) and spectrin β II (also designated fodrin β) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Activation of calpain results in the breakdown of spectrin α II, a neuronal cytoskeleton protein.
Background References
1. Yamamoto K et al. The neuroprotective effect of latanoprost acts via klotho-mediated suppression of calpain activation after optic nerve transection. J Neurochem 140:495-508 (2017).
2. Liu M et al. Heme-mediated apoptosis and fusion damage in BeWo trophoblast cells. Sci Rep 6:36193 (2016).
Sequence Similarity
Belongs to the spectrin family.
Post-translational Modification
Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Subcellular Location
Cytoskeleton.
Synonyms
(ALPHA)II-SPECTRIN antibody
Alpha II Spectrin antibody
Alpha-II spectrin antibodybrain antibody
EIEE5 antibody
FLJ17738 antibody
FLJ44613 antibody
Fodrin alpha chain antibody
Fodrin, alpha antibody
NEAS antibody
Non erythrocytic spectrin alpha antibody
ExpandImages
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Western blot analysis of Alpha Fodrin on different lysates with Rabbit anti-Alpha Fodrin antibody (ET1706-44) at 1/500 dilution.
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: Rat brain tissue lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (20 µg/Lane)
Predicted band size: 285 kDa
Observed band size: 285/150 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-44) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Alpha Fodrin antibody (ET1706-44) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-44) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Alpha Fodrin antibody (ET1706-44) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-44) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Alpha Fodrin antibody (ET1706-44) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-44) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"