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Alcohol Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JG82-31]

Usd: 385

Specification

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Catalog# ET7108-64

Alcohol Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JG82-31]

Application

  • WB

  • IHC-P

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Alcohol Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JG82-31]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Alcohol Dehydrogenase aa 2-107 / 375.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, IF-Tissue

Target Molecular Weight

40 kDa

Positive Control

Rat liver tissue lysates, HepG2, human liver tissue, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

JG82-31

RRID

AB_3070860

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

Target

Function

The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha (ADH1A), beta (ADH1B) and gamma (ADH1C) subunits are tandemly organized on chromosome 4q22 as a gene cluster. The alpha form of ADH is monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. The genes encoding beta and gamma subunits, however, are polymorphic and strongly expressed in adult livers. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH in the liver is the removal of ethanol formed by microorganisms in the intestinal tract.

Background References

1. Gibbons B J et al. Structure of three class I human alcohol dehydrogenases complexed with isoenzyme specific formamide inhibitors. Biochemistry 43:12555-12562 (2004).

2. Niederhut M S et al. Three-dimensional structures of the three human class I alcohol dehydrogenases. Protein Sci 10:697-706 (2001).

Sequence Similarity

Belongs to the zinc-containing alcohol dehydrogenase family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P07327 Human

SwissProt: P00329 Mouse

SwissProt: P06757 Rat

Synonyms

ADH alpha subunit antibody

ADH antibody

ADH1 antibody

ADH1A antibody

ADH1A_HUMAN antibody

Alcohol dehydrogenase 1 (class I), alpha polypeptide antibody

Alcohol dehydrogenase 1 antibody

Alcohol dehydrogenase 1A (class I), alpha polypeptide antibody

Alcohol dehydrogenase 1A antibody

Alcohol dehydrogenase subunit alpha antibody

Expand

Images

  • Immunocytochemistry analysis of HepG2 cells labeling Alcohol Dehydrogenase with Rabbit anti-Alcohol Dehydrogenase antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alcohol Dehydrogenase antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of HepG2 cells labeling Alcohol Dehydrogenase with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of Alcohol Dehydrogenase on different lysates with Rabbit anti-Alcohol Dehydrogenase antibody (<a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>) at 1/1,000 dilution.<br /><br />Lane 1: mouse liver tissue lysate <br />Lane 2: rat liver tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br />Exposure time: 4 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7108-64" style="font-weight: bold;text-decoration: underline;">ET7108-64</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 39.9 kDa<br />Observed band size: 40 kDa

    Western blot analysis of Alcohol Dehydrogenase on different lysates with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/1,000 dilution.

    Lane 1: mouse liver tissue lysate
    Lane 2: rat liver tissue lysate

    Lysates/proteins at 40 µg/Lane.
    Exposure time: 4 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7108-64, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 39.9 kDa
    Observed band size: 40 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"