Acetyl CoA synthetase Recombinant Rabbit Monoclonal Antibody [JF0917]
Catalog# ET1702-21
Acetyl CoA synthetase Recombinant Rabbit Monoclonal Antibody [JF0917]
-
WB
-
IF-Cell
-
IF-Tissue
-
Human
-
Mouse
-
Rat
-
Green monkey
-
unconjugated
Overview
Product Name
Acetyl CoA synthetase Recombinant Rabbit Monoclonal Antibody [JF0917]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Acetyl CoA synthetase aa 101-145 / 701.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell, IF-Tissue
Molecular Weight
Predicted band size: 79 kDa
Positive Control
HepG2 cell lysate, L6 cell lysate, COS-1 cell lysate, RH-35, SW480.
Conjugation
unconjugated
Clone Number
JF0917
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:2,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
Target
Function
ACSS2 (acyl-CoA synthetase short-chain family member 2), also known as ACAS2, ACS, ACSA or AceCS, is a 701 amino acid cytoplasmic protein that belongs to the ATP-dependent AMP-binding enzyme family. Existing as a monomer, ACSS2 functions to catalyze the ATP-dependent activation of acetate, a reaction that yields acetyl-CoA for use in energy generation and lipid synthesis. ACSS2 expression, which is highest in liver and kidney tissue, is regulated by the presence of unsaturated fatty acids and sterol regulatory element-binding proteins (SREBPs). Human ACSS2 exists as two alternatively spliced isoforms and shares 93% sequence identity with its mouse counterpart, suggesting a conserved role between species.
Background References
1. Xu M et al. An acetate switch regulates stress erythropoiesis. Nat Med 20:1018-26 (2014).
2. Soliman ML et al. Acetate reduces microglia inflammatory signaling in vitro. J Neurochem 123:555-67 (2012).
Sequence Similarity
Belongs to the ATP-dependent AMP-binding enzyme family.
Post-translational Modification
Reversibly acetylated at Lys-661 (By similarity). The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3 (By similarity).
Subcellular Location
Cytoplasm, Nucleus.
UNIPROT
Synonyms
ACAS2 antibody
AceCS antibody
Acetate CoA ligase antibody
Acetate thiokinase antibody
Acetate--CoA ligase antibody
Acetyl CoA synthetase antibody
Acetyl Coenzyme A synthetase 2 (ADP forming) antibody
Acetyl coenzyme A synthetase cytoplasmic antibody
Acetyl-CoA synthetase antibody
Acetyl-coenzyme A synthetase antibody
ExpandImages
-
Western blot analysis of Acetyl CoA synthetase on different lysates with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: L6 cell lysate
Lane 3: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Acetyl CoA synthetase on different lysates with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si Acetyl CoA synthetase cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of Acetyl CoA synthetase in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
-
ICC staining of Acetyl CoA synthetase in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
-
Immunocytochemistry analysis of HEPG2 cells labeling Acetyl CoA synthetase with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
"Immunocytochemistry analysis of L6 cells labeling Acetyl CoA synthetase with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Acetyl CoA synthetase antibody (ET1702-21) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution."
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"