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Liver Arginase Recombinant Rabbit Monoclonal Antibody [SY09-06]

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Specification

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Catalog# ET1605-8

Liver Arginase Recombinant Rabbit Monoclonal Antibody [SY09-06]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Liver Arginase Recombinant Rabbit Monoclonal Antibody [SY09-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human ARG1 aa 20-60.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate, RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate.

Conjugation

unconjugated

Clone Number

SY09-06

RRID

AB_3069721

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Arginase I (also designated liver-type arginase), which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea. The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.

Background References

1. Chugh, D. et al. 2015. Alterations in Brain Inflammation, Synaptic Proteins, and Adult Hippocampal Neurogenesis during Epileptogenesis in Mice Lacking Synapsin2. PloS one. 10: e0132366.

2. Teplova, I. et al. 2013. ATG proteins mediate efferocytosis and suppress inflammation in mammary involution. Autophagy. 9: 459-75.

Sequence Similarity

Belongs to the arginase family.

Tissue Specificity

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Subcellular Location

Cytoplasm,Cytoplasmic granule.

UNIPROT

SwissProt: P05089 Human

SwissProt: Q61176 Mouse

SwissProt: P07824 Rat

Synonyms

arg1 antibody

ARGI1_HUMAN antibody

Arginase-1 antibody

Liver-type arginase antibody

Type I arginase antibody

Images

  • Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (<a href="/products/ET1605-8" style="font-weight: bold;text-decoration: underline;">ET1605-8</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human liver tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Rat liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1605-8" style="font-weight: bold;text-decoration: underline;">ET1605-8</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (ET1605-8) at 1/2,000 dilution.

    Lane 1: Human liver tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Rat liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 37 kDa

    Exposure time: 30 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (<a href="/products/ET1605-8" style="font-weight: bold;text-decoration: underline;">ET1605-8</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate<br />Lane 3: RAW264.7 cell lysate<br />Lane 4: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1605-8" style="font-weight: bold;text-decoration: underline;">ET1605-8</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (ET1605-8) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate
    Lane 3: RAW264.7 cell lysate
    Lane 4: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 37 kDa

    Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation