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ACTL6A Recombinant Rabbit Monoclonal Antibody [JU40-32]

Usd: 385

Specification

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Catalog# ET7106-60

ACTL6A Recombinant Rabbit Monoclonal Antibody [JU40-32]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

ACTL6A Recombinant Rabbit Monoclonal Antibody [JU40-32]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human ACTL6A aa 185-326 .

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Molecular Weight

47 kDa

Positive Control

Jurkat cell lysates, mouse brain tissue lysates, LOVO, PC-3M, human spleen tissue, mouse brain tissue, mouse hippocampus tissue.

Conjugation

unconjugated

Clone Number

JU40-32

RRID

AB_3070680

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:100

  • IF-Tissue

  • 1:50-1:100

  • IHC-P

  • 1:50-1:1,000

  • IP

  • 1:10-1:50

Target

Function

ACTL6A also named BAF53. The SWI/SNF complex regulates gene expression via ATP-dependent chromatin remodeling. Brm (SNF2-a), Brg-1 (SNF2-b), Ini1 (integrase interactor 1, SNF5), BAF53 (ARPnβ), BAF57, BAF155 (SRG3), and BAF170 make up the functional core. BAF53 homologues from yeast to humans contain a conserved N-terminal motif, which contains residues at serine 2 and tyrosine 6, which play important roles in BAF53 activity. The BAF53 protein shuttles between the nucleus and cytoplasm. BAF53 also forms a complex with TIP49 and TIP48, which mediates c-Myc oncogenic activity.

Background References

1. Euskirchen G et al. SWI/SNF chromatin-remodeling factors: multiscale analyses and diverse functions. J Biol Chem 287:30897-30905 (2012).

2. Doyon Y et al. Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans. Mol Cell Biol 24:1884-1896 (2004).

Sequence Similarity

Belongs to the actin family.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O96019 Human

SwissProt: Q9Z2N8 Mouse

Synonyms

53 kDa BRG1 associated factor A antibody

53 kDa BRG1-associated factor A antibody

ACL6A_HUMAN antibody

Actin like 6A antibody

Actin like protein 6A antibody

Actin related protein 4 antibody

Actin related protein antibody

Actin related protein Baf53a antibody

Actin-like protein 6A antibody

Actin-related protein Baf53a antibody

Expand

Images

  • Western blot analysis of ACTL6A on Jurkat cell(1) and mouse brain tissue(2) lysate using anti-ACTL6A antibody at 1/500 dilution.

    Western blot analysis of ACTL6A on Jurkat cell(1) and mouse brain tissue(2) lysate using anti-ACTL6A antibody at 1/500 dilution.

  • ICC staining ACTL6A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ACTL6A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ACTL6A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ACTL6A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACTL6A antibody (<a href="/products/ET7106-60" style="font-weight: bold;text-decoration: underline;">ET7106-60</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-60" style="font-weight: bold;text-decoration: underline;">ET7106-60</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACTL6A antibody (ET7106-60) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-ACTL6A antibody (<a href="/products/ET7106-60" style="font-weight: bold;text-decoration: underline;">ET7106-60</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-60" style="font-weight: bold;text-decoration: underline;">ET7106-60</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-ACTL6A antibody (ET7106-60) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"