Applications
-
ICC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
ROBO1 Antibody (ER1706-24)
Immunogen
Peptide
Host
Rabbit
Positive Control
Hela, MCF-7, A549, human liver cancer tissue, human breast tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
180 kDa
Isotype
IgG
APPLICATION DILUTION
-
ICC
-
1:100-1:500
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
ROBO1
SYNONYMS
Deleted in U twenty twenty antibody; DUTT 1 antibody; DUTT1 antibody; FLJ21882 antibody; H Robo 1 antibody; H-Robo-1 antibody; hRobo 1 antibody; Robo 1 antibody; Robo1 antibody; ROBO1_HUMAN antibody; Roundabout 1 antibody; Roundabout axon guidance receptor homolog 1 antibody; Roundabout homolog 1 antibody; Roundabout homolog1 precurser antibody; Roundabout1 antibody; SAX 3 antibody; SAX3 antibody
SEQUENCE SIMILARITIES
Belongs to the immunoglobulin superfamily. ROBO family.
TISSUE SPECIFICITY
Widely expressed, with exception of kidney.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated. May be deubiquitinated by USP33.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Specific cells in the midline separate the left and right halves of the central nervous system and play many roles in guiding the growth cone behavior. In vertebrate spinal cord, insect abdominal nerves and nematodes, midline cells produce induced cues, such as nectins and slits, respectively, as attractants and repellents. respectively. These cells can serve as gatekeepers to prevent axons from passing through the midline and to guide the growth cone in response to the switching of lead cues beyond the crossing. One such gatherer, Robo, is an axon guidance receptor that defines a new subfamily of proteins from fruit flies to mammalian conserved Ig superfamily proteins. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous manner Non-intersecting axons express high levels of Robo and cross axons to express low levels of Robo and then reach midline and high levels of crossover. Robo1 and Robo2 are two human homologs of Drosophila megalopolis. Robo1 is also homologous to the C. elegans gene sax3, while Robo2 is homologous to zebrafish genes.