B7-H4(VTCN1) Rabbit Polyclonal Antibody
Catalog# R1512-1
B7-H4(VTCN1) Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
B7-H4(VTCN1) Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human B7-H4 aa 65-226 / 282.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
SK-Br-3 cell lysate, human liver tissue lysate, mouse testis tissue lysate, mouse spleen tissue lysate, Hela, MCF-7, SK-Br-3, human tonsil tissue, human lung cancer tissue, Jurkat.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:200
Target
Function
T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4, also designated VTCN1), belongs to the B7 Immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells supresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.
Background References
1. Huang H et al. Clinical significance of the B7-H4 as a novel prognostic marker in breast cancer. Gene 623:24-28 (2017).
2. Bregar A et al. Characterization of immune regulatory molecules B7-H4 and PD-L1 in low and high grade endometrial tumors.Gynecol Oncol 145(3):446-452 (2017).
Sequence Similarity
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Tissue Specificity
Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.
Post-translational Modification
N-glycosylated.
Subcellular Location
Cell membrane.
Synonyms
B7 family member, H4 antibody
B7 H4 antibody
B7 homolog 4 antibody
B7 superfamily member 1 antibody
B7 superfamily, member 1 antibody
B7-H4 antibody
B7h.5 antibody
B7h4 antibody
B7S1 antibody
B7x antibody
ExpandImages
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Western blot analysis of B7-H4 on different lysate using anti-B7-H4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SK-Br-3 cell lysate
Lane 2: Human liver tissue lysate
Lane 3: Mouse testis tissue lysate
Lane 4: Mouse spleen tissue lysate -
ICC staining B7-H4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining B7-H4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining B7-H4 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- B7-H4 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-B7-H4 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
B7H4 Recombinant Rabbit Monoclonal Antibody [PSH03-65]
Application: WB,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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