Bad Rabbit Polyclonal Antibody (R1511-32)

UNIPROT #

Q92934

PROTEIN NAME

Bad

SYNONYMS

AI325008 antibody;BAD antibody;BAD_HUMAN antibody;BBC 2 antibody;BBC2 antibody;BBC6 antibody;Bcl 2 Antagonist of Cell Death antibody;Bcl 2 Binding Component 6 antibody;BCL X / BCL 2 Binding Protein antibody;BCL X Binding Protein antibody;Bcl XL/Bcl 2 Associated Death Promoter antibody;Bcl-2-binding component 6 antibody;Bcl-2-like protein 8 antibody;Bcl-XL/Bcl-2-associated death promoter antibody;Bcl2 antagonist of cell death antibody;BCL2 antagonist of cell death protein antibody;BCL2 associated agonist of cell death antibody;Bcl2 Associated Death Promoter antibody;BCL2 binding component 6 antibody;BCL2 binding protein antibody;Bcl2 Like 8 Protein antibody;Bcl2-L-8 antibody;BCL2L8 antibody;Proapoptotic BH3 Only Protein antibody

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

TISSUE SPECIFICITY

Expressed in a wide variety of tissues.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.; Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.

SUBCELLULAR LOCATION

Mitochondrion outer membrane, Cytoplasm

FUNCTION

The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2 limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2 and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL:Bax complexes thereby causing restoration of Bax-mediated apoptosis.