CRISPR-Cas9 Rabbit Polyclonal Antibody
Catalog# R1504-1
CRISPR-Cas9 Rabbit Polyclonal Antibody
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WB
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ELISA
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IF-Cell
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Staphylococcus aureus
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unconjugated
Overview
Product Name
CRISPR-Cas9 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within CRISPR-Cas9 aa 54-141.
Species Reactivity
Staphylococcus aureus
Validated Applications
WB, ELISA, IF-Cell
Target Molecular Weight
Predicted band size: 158 kDa
Positive Control
CRISPR-Cas9 transfected 293 cells.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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ELISA
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1:10,000
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IF-Cell
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1:200-1:500
Target
Function
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.
Background References
1. Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12.
2. Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
Synonyms
Cas9 antibody
CRISPR-associated endonuclease Cas9/Csn1 antibody
CRISPR-Cas9/Csn1 antibody
csn1 antibody
SpyCas9 antibody
Images
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☑ Cell treatment (CT)
Western blot analysis on CRISPR-Cas9 non transtected 293 cells (-) and transfected 293 cells (+). -
☑ Cell treatment (CT)
Immunofluorescence analysis of CRISPR-Cas9 transfected 293 cells. The cell expressing CRISPR-Cas9 can be detected using rabbit anti- CRISPR-Cas9 (1/500) followed by FITC (1/1,000) goat anti-rabbit IgG.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CRISPR-Cas9 Mouse Monoclonal Antibody [8-A6-6]
Application: WB,IF-Cell
Reactivity: Staphylococcus aureus
Conjugate: unconjugated
