LINE-1 ORF1p Rabbit Polyclonal Antibody
Catalog# R1411-1
LINE-1 ORF1p Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Mouse
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unconjugated
Overview
Product Name
LINE-1 ORF1p Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse LINE-1 ORF1p aa 308-357 / 357.
Species Reactivity
Mouse
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 41 kDa
Positive Control
Mouse testis tissue lyaste, Mouse ovary tissue lyaste.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50-1:100
Target
Function
The L1 (LINE 1) retrotransposable element encodes two proteins, ORF1p and ORF2p. Mouse ORF1p, a coiled-coil-mediated trimer of 42-kDa monomers. It may function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.
Background References
1. Martin S.L., Bushman F.D. Mol. Cell. "Nucleic acid chaperone activity of the ORF1 protein from the mouse LINE-1 retrotransposon." Biol. 21:467-475(2001)
2. "Trimeric structure for an essential protein in L1 retrotransposition." Martin S.L., Branciforte D., Keller D., Bain D.L. Proc. Natl. Acad. Sci. U.S.A. 100:13815-13820(2003)
3. "Identification and solution structure of a highly conserved C-terminal domain within ORF1p required for retrotransposition of long interspersed nuclear element-1." Januszyk K., Li P.W., Villareal V., Branciforte D., Wu H., Xie Y., Feigon J., Loo J.A., Martin S.L., Clubb R.T. J. Biol. Chem. 282:24893-24904(2007)
Sequence Similarity
Belongs to the transposase 22 family.
Tissue Specificity
Expressed in meiotic spermatocytes and in the cerebellum (at protein level).
Post-translational Modification
Polyubiquitinated, probably by UBR2, which induces its degradation.
Subcellular Location
Cytoplasm, nucleus.
UNIPROT
Synonyms
L12R1_HUMAN antibody
loh12cr1 antibody
Loss of heterozygosity 12 chromosomal region 1 protein antibody
Images
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Western blot analysis on tissue lysates using anti- L1ORF1p rabbit polyclonal antibodies.
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ICC staining L1ORF1p in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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