PTBP1 Rabbit Polyclonal Antibody
Catalog# R1403-2
PTBP1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PTBP1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to Human PTBP1 aa 1-50 / 531.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 60 kDa
Positive Control
Jurkat, Daudi, A549, rat heart, PC12, NIH/3T3, F9, Hela , mouse testis
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:200
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IF-Cell
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1:200
Target
Function
Polypyrimidine tract-binding protein 1 is a protein that in humans is encoded by the PTBP1 gene. It plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. PTBP1 activates exon skipping of its own pre-mRNA during muscle cell differentiation. It binds to the polypyrimidine tract of introns and may promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. PTBP1 may promote the binding of U2 snRNP to pre-mRNA. It cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10.
Background References
1. "Structure of PTB bound to RNA: specific binding and implications for splicing regulation." Oberstrass F.C., Auweter S.D., Erat M., Hargous Y., Henning A., Wenter P., Reymond L., Amir-Ahmady B., Pitsch S., Black D.L., Allain F.H. Science 309:2054-2057(2005)
2. "Exon selection in alpha-tropomyosin mRNA is regulated by the antagonistic action of RBM4 and PTB." Lin J.C., Tarn W.Y. Mol. Cell. Biol. 25:10111-10121(2005)
3. "RBM4 down-regulates PTB and antagonizes its activity in muscle cell-specific alternative splicing." Lin J.C., Tarn W.Y. J. Cell Biol. 193:509-520(2011)
Subcellular Location
Nucleus
Synonyms
57 kDa RNA binding protein PPTB 1 antibody
57 kDa RNA-binding protein PPTB-1 antibody
Heterogeneous nuclear ribonucleoprotein I antibody
Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody
hnRNP I antibody
HNRNP-I antibody
HNRNPI antibody
HNRPI antibody
MGC10830 antibody
MGC8461 antibody
ExpandImages
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Western blot analysis on cell lysates using anti- PTBP1 rabbit polyclonal antibodies.
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ICC staining PTBP1 in Hela cells (green). Cells were fixed in paraformaldehyde.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- PTBP1 rabbit polyclonal antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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