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PRDM14 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1310-4

PRDM14 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PRDM14 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human PRDM14 aa 1-250 / 571.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

~64kDa

Positive Control

Human embryonic stem cells, human lung, MCF-7

Conjugation

unconjugated

RRID

AB_3073253

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:100

Target

Function

PR domain zinc finger protein 14 is a transcription factor that has both positive and negative roles on transcription. It is expressed in embryonic stem cells and tends to be overexpressed in breast cancer. PRDM14 is required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. It may play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. PRDM14 directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer.

Background References

1. "Gene amplification and overexpression of PRDM14 in breast cancers." Nishikawa N., Toyota M., Suzuki H., Honma T., Fujikane T., Ohmura T., Nishidate T., Ohe-Toyota M., Maruyama R., Sonoda T., Sasaki Y., Urano T., Imai K., Hirata K., Tokino T. Cancer Res. 67:9649-9657(2007)

2. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011)

Sequence Similarity

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Tissue Specificity

Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level).

Subcellular Location

Nucleus

UNIPROT

SwissProt: Q9GZV8 Human

Synonyms

MGC59730 antibody

PFM 11 antibody

PFM11 antibody

PR domain containing 14 antibody

PR domain zinc finger protein 14 antibody

PR domain-containing protein 14 antibody

PRD14_HUMAN antibody

PRDM 14 antibody

PRDM14 antibody

Images

  • Western blot analysis on hES cell lysates using anti-PRDM14 rabbit polyclonal antibodies.

    Western blot analysis on hES cell lysates using anti-PRDM14 rabbit polyclonal antibodies.

  • ICC staining PRDM14 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    ICC staining PRDM14 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- PRDM14 rabbit polyclonal antibody.

    Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- PRDM14 rabbit polyclonal antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"