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PLCG2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1512-4

PLCG2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PLCG2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human PLCG2 aa 1200-1265.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

148 kDa

Positive Control

Raji cell lysate, Daudi cell lysate, AGS, human tonsil tissue, Daudi.

Conjugation

unconjugated

RRID

AB_3073397

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:100

  • IHC-P

  • 1:100-1:400

  • FC

  • 1:50-1:100

Target

Function

Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC e. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC γ2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR γ chain), which bind to its C-terminal SH2 domain. PLC γ1 associates with Syk in B cells, but PLC γ2 does not associate with Syk in platelets. The C-terminal SH2 domain is involved in the regulation of PLC γ2. In addition, Btk can induce PLC γ2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes.

Background References

1. Baek JM et al. Nicotinamide phosphoribosyltransferase inhibits receptor activator of nuclear factor-κB ligand-induced osteoclast differentiation in vitro. Mol Med Rep 15(2):784-792 (2017).

Subcellular Location

Cytosol, Autolysosome.

UNIPROT

SwissProt: P16885 Human

Synonyms

1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody

1-phosphatidylinositol-4 antibody

5-bisphosphate phosphodiesterase gamma-2 antibody

EC 3.1.4.11 antibody

Phosphoinositide phospholipase C antibody

Phosphoinositide phospholipase C-gamma-2 antibody

Phospholipase C gamma 2 antibody

Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody

Phospholipase C-gamma-2 antibody

Phospholipase C-IV antibody

Expand

Images

  • Western blot analysis of PLCG2 on different lysates with Rabbit anti-PLCG2 antibody (<a href="/products/R1512-4" style="font-weight: bold;text-decoration: underline;">R1512-4</a>) at 1/1,000 dilution.<br /><br />Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate<br />Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 6 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1512-4" style="font-weight: bold;text-decoration: underline;">R1512-4</a>, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 148 kDa<br />Observed band size: 148 kDa

    Western blot analysis of PLCG2 on different lysates with Rabbit anti-PLCG2 antibody (R1512-4) at 1/1,000 dilution.

    Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate
    Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 6 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1512-4, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 148 kDa
    Observed band size: 148 kDa

  • ICC staining of PLCG2 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/R1512-4" style="font-weight: bold;text-decoration: underline;">R1512-4</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of PLCG2 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1512-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PLCG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1512-4" style="font-weight: bold;text-decoration: underline;">R1512-4</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PLCG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-4, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of PLCG2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/R1512-4" style="font-weight: bold;text-decoration: underline;">R1512-4</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PLCG2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (R1512-4, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

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PLCG 2 Recombinant Rabbit Monoclonal Antibody [JE39-12]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated