NaV1.7 Mouse Monoclonal Antibody [1D6]
Catalog# EM1701-35
NaV1.7 Mouse Monoclonal Antibody [1D6]
-
IHC-P
-
IF-Cell
-
FC
-
WB
-
Human
-
unconjugated
Overview
Product Name
NaV1.7 Mouse Monoclonal Antibody [1D6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human NaV17 aa 1,577-1,626 / 1,988.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Cell, FC, WB
Molecular Weight
Predicted band size: 226 kDa
Positive Control
A549, Hela, human colon cancer tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
1D6
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein A affinity purified.
Application Dilution
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:200
-
WB
-
1:500
Target
Function
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain.
Background References
1. Henningsen K et al. Candidate hippocampal biomarkers of susceptibility and resilience to stress in a rat model of depression. Mol Cell Proteomics 11:M111.016428 (2012).
2. Weiss J et al. Loss-of-function mutations in sodium channel Nav1.7 cause anosmia. Nature 472:186-90 (2011).
Sequence Similarity
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
Tissue Specificity
Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.
Post-translational Modification
Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.; Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.
Subcellular Location
Cell membrane, Cell projection.
UNIPROT
Synonyms
ETHA antibody
GEFSP7 antibody
hNE Na antibody
hNE-Na antibody
hNENa antibody
NE NA antibody
NENA antibody
Neuroendocrine sodium channel antibody
Peripheral sodium channel 1 antibody
PN1 antibody
ExpandImages
-
ICC staining NaV1.7 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining NaV1.7 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"