Mcur1 Rabbit Polyclonal Antibody
Catalog# ER1902-30
Mcur1 Rabbit Polyclonal Antibody
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WB
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Mouse
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unconjugated
Overview
Product Name
Mcur1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse Mcur1 79-128 / 340.
Species Reactivity
Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 38 kDa
Positive Control
Mouse brain tissue lysates.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:500
Target
Function
Ca2+ flux across the mitochondrial inner membrane regulates bioenergetics, cytoplasmic Ca2+ signals and activation of cell death pathways. Mitochondrial Ca2+ uptake occurs at regions of close apposition with intracellular Ca2+ release sites driven by the inner membrane voltage generated by oxidative phosphorylation and mediated by a Ca2+ selective ion channel called the uniporter. Mitochondrial calcium uniporter (MCU) was recently identified as an ion-conducting pore. MCUR1 (mitochondrial calcium uniporter regulator 1), belongs to the CCDC90 family, an integral membrane protein required for MCU-dependent mitochondrial Ca2+ uptake. MCUR1 binds to MCU and regulates ruthenium-red-sensitive MCU-dependent Ca2+ uptake. MCUR1 contains two transmembrane helices, where the N- and the short C-termini are exposed to the inter-membrane space. MCUR1 is ubiquitously expressed in mammalian tissues including: skeletal muscle, brain, heart, lung, liver, kidney, large intenstine and spleen. It was found that cells depleted of MCUR1 have abnormal bioenergetic properties similar to those observed in cells in which Ca2+ uptake is blocked. Thus, MCUR1 is necessary for MCU to efficiently promote mitochondrial Ca2+ uptake and to ensure normal mitochondrial bioenergetics.
Background References
1. Mallilankaraman K.et.al.MCUR1 is an essential component of mitochondrial Ca(2+) uptake that regulates cellular metabolism.Nat. Cell Biol. 14:1336-1343(2012).
Sequence Similarity
Belongs to the CCDC90 family.
Subcellular Location
Mitochondrion inner membrane。
UNIPROT
Synonyms
Mitochondrial calcium uniporter regulator antibody
MCU regulator 1 antibody
Coiled-coil domain-containing protein 90A, mitochondrial antibody
Mcur1 antibody
Ccdc90a antibody
Images
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Western blot analysis of Mcur1 on mouse brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"