Monoamine Oxidase A / MAO-A Mouse Monoclonal Antibody [5D1]
Catalog# EM1701-54
Monoamine Oxidase A / MAO-A Mouse Monoclonal Antibody [5D1]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Monoamine Oxidase A / MAO-A Mouse Monoclonal Antibody [5D1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human MAO-A aa 222-517 / 527.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 60 kDa
Positive Control
SH-SY5Y cell lysate, SiHa cell lysate, rat brain tissue lysate, mouse brain tissue lysate, SH-SY-5Y.
Conjugation
unconjugated
Clone Number
5D1
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
Target
Function
This gene is one of two neighboring gene family members that encode mitochondrial enzymes which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. Mutation of this gene results in Brunner syndrome. Has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues.This gene has also been associated with a variety of other psychiatric disorders, including antisocial behavior. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Background References
1. De Colibus L. et al. Three-dimensional structure of human monoamine oxidase A (MAO A): relation to the structures of rat MAO A and human MAO B. Proc. Natl. Acad. Sci. U.S.A. 102:12684-12689(2005).
2. Sjoeblom T. al. The consensus coding sequences of human breast and colorectal cancers. Science 314:268-274(2006).
Sequence Similarity
Belongs to the flavin monoamine oxidase family.
Tissue Specificity
Heart, liver, duodenum, blood vessels and kidney.
Subcellular Location
Mitochondrion outer membrane.
Synonyms
Amine oxidase [flavin containing] A antibody
Amine oxidase [flavin-containing] A antibody
AOFA antibody
AOFA_HUMAN antibody
EC 1.4.3.4 antibody
MAO A antibody
MAO-A antibody
maoA antibody
Monoamine oxidase A antibody
Monoamine oxidase type A antibody
Images
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Western blot analysis of Monoamine Oxidase A / MAO-A on different lysates with Mouse anti-Monoamine Oxidase A / MAO-A antibody (EM1701-54) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: SiHa cell lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-2: 46 seconds; Lane 3-4: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining Monoamine Oxidase A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Monoamine Oxidase A / MAO-A Recombinant Mouse Monoclonal Antibody [5D1-R]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Monoamine Oxidase A / MAO-A Recombinant Rabbit Monoclonal Antibody [JE39-96]
Application: WB,IHC-P,IF-Tissue,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
