FAM234A Mouse Monoclonal Antibody [A5-A10]
Catalog# M1010-1
FAM234A Mouse Monoclonal Antibody [A5-A10]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
FAM234A Mouse Monoclonal Antibody [A5-A10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human FAM234A aa 300-552.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 60 kDa
Positive Control
SiHa cell lysate, SH-SY5Y cell lysate, NIH:OVCAR-3 cell lysate, A549 cell lysate, Neuro-2a cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
A5-A10
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
Target
Function
Protein ITFG3 also known as family with sequence similarity 234 member A (FAM234A) is a protein that in humans is encoded by the ITFG3 gene. Here, the gene is explored as encoded by mRNA found in Homo sapiens. The FAM234A gene is conserved in mice, rats, chickens, zebrafish, dogs, cows, frogs, chimpanzees, and rhesus monkeys. Orthologs of the gene can be found in at least 220 organisms including the tropical clawed frog, pandas, and Chinese hamsters. The gene is located at 16p13.3 and has a total of 19 exons. The mRNA has a total of 3224 bp and the protein has 552 aa.The molecular mass of the protein produced by this gene is 59660 Da. It is expressed in at least 27 tissue types in humans, with the greatest presence in the duodenum, fat, small intestine, and heart.
Background References
1. Ota T. et. al. Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. 36:40-45(2004).
Subcellular Location
Membrane.
Synonyms
Protein ITFG3 antibody
Protein FAM234A antibody
C16orf9 antibody
ITFG3 antibody
FAM234A antibody
Images
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Western blot analysis of FAM234A on different lysates with Mouse anti-FAM234A antibody (M1010-1) at 1/2,000 dilution.
Lane 1: SiHa cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: NIH:OVCAR-3 cell lysate
Lane 4: A549 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 55 kDa
Exposure time: 2 minutes 5 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1010-1) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"