LRRK1 Mouse Monoclonal Antibody [5B1]
Catalog# M1701-14
LRRK1 Mouse Monoclonal Antibody [5B1]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
LRRK1 Mouse Monoclonal Antibody [5B1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within mouse LRRK1 aa 1-164 / 2,015.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 225kDa, additional band 60kDa
Positive Control
A549, mouse brain tissue, mouse kidney tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
5B1
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:50-1:100
Target
Function
Leucine-rich repeat serine/threonine-protein kinase 1 is an enzyme that in humans is encoded by the LRRK1 gene. Formation of LRRK1:EGF-R complexes result in LRRK cellular translocation: LRRK1 traffics together with EGF-R to endosomes upon stimulation of the receptor with EGF. LRRK1 is related to the familial Parkinsonism gene product Park8/LRRK2, and contains a Ras of complex proteins (ROC) GTPase domain and a MAPKKK-like kinase domain. LRRK1 participates in the intercellular trafficking of the epidermal growth factor (EGF) receptor. LRRK1 is activated by the sequential phosphorylation of 2 mitotic kinases, PLK1 and CDK1. Importantly, this PLK1-dependent activation of LRRK1 is required for the regulation of mitotic spindle orientation.LRRK1 regulates spindle orientation in a manner dependent on its kinase activity. Furthermore, the spindle misorientation caused by PLK1 inactivation is rescued by expression of the phospho-mimicking mutant LRRK1(S1790D). LRRK1 regulates spindle orientation downstream of PLK1.
Background References
1. Guo L et al. Identification of a novel LRRK1 mutation in a family with osteosclerotic metaphyseal dysplasia.J Hum Genet 62(3):437-441 (2017).
2. Sejwal K et al. Cryo-EM analysis of homodimeric full-length LRRK2 and LRRK1 protein complexes.Sci Rep 7(1):8667 (2017).
Sequence Similarity
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ROCO subfamily.
Subcellular Location
Cytoplasm.
Synonyms
KIAA1790 antibody
Leucine rich repeat serine/threonine protein kinase 1 antibody
Leucine-rich repeat serine/threonine-protein kinase 1 antibody
LRRK 1 antibody
Lrrk1 antibody
LRRK1_HUMAN antibody
RIPK6 antibody
Roco1 antibody
Images
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Western blot analysis of LRRK1 on A549 lysate using anti-LRRK1 antibody at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"