LASS2 Mouse Monoclonal Antibody [B3-2]
Catalog# M1006-6
LASS2 Mouse Monoclonal Antibody [B3-2]
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WB
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IF-Cell
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IHC-P
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Human
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Zebrafish
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unconjugated
Overview
Product Name
LASS2 Mouse Monoclonal Antibody [B3-2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human LASS2 aa 331-380 / 380.
Species Reactivity
Human, Zebrafish
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 45 kDa
Positive Control
K562-AO2 cell lysates, hybrid fish (crucian-carp) brain tissue lysates, human brain tissue, HepG2 cells.
Conjugation
unconjugated
Clone Number
B3-2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500~1:1,000
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IF-Cell
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1:50~1:200
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IHC-P
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1:50~1:200
Target
Function
The LAG1 gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. Expression of LASS2 in hepatocellular carcinoma cell lines suppresses the growth of cancer cells.The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described.
Background References
1. "Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1."Pan H., Qin W.-X., Huo K.-K., Wan D.-F., Yu Y., Xu Z.-G., Hu Q.-D., Gu K.T., Zhou X.-M., Jiang H.-Q., Zhang P.-P., Huang Y., Li Y.-Y., Gu J.-R.Genomics 77:58-64(2001)
2. “Expression of LASS2 controlled by LAG1 or ADH1 promoters cannot functionally complement Lag1p” Yu, Y., Lu, H., Pan, H., Ma, J.H., Ding, Z.J. and Li, Y.Y.. Microbiol. Res. 161: 203-211.(2006)
Tissue Specificity
Expressed in kidney, liver, brain, heart, placenta and lung.
Post-translational Modification
Acetylated. Deacetylation by SIRT3 increases enzyme activity and promotes mitochondrial ceramide accumulation.; Phosphorylated at the C-terminus by CK2, leading to increase the ceramide synthase activity.
Subcellular Location
Endoplasmic reticulum membrane
Synonyms
Ceramide synthase 2 antibody
CerS 2 antibody
CerS2 antibody
CERS2_HUMAN antibody
FLJ10243 antibody
L3 antibody
LAG1 homolog ceramide synthase 2 antibody
LAG1 longevity assurance 2 antibody
LAG1 longevity assurance homolog 2 antibody
LASS 2 antibody
ExpandImages
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Western blot analysis on K562-AO2 cell lysates using anti-lass2 monoclonal antibody.
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Western blot analysis of LASS2 in on hybrid fish (crucian-carp) brain tissue lysate using anti-LASS2 antibody at 1/500 dilution.
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ICC staining LASS2 (Green) in HepG2 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-LASS2 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"