Ku80 Rabbit Polyclonal Antibody
Catalog# R1511-17
Ku80 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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IP
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Human
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Mouse
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unconjugated
Overview
Product Name
Ku80 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Ku80 aa 51-104.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P, IP
Molecular Weight
Predicted band size: 83 kDa
Positive Control
A549, Hela, MCF-7, human liver tissue lysate, human colon cancer tissue, human tonsil tissue, human breast cancer tissue, human kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2.000
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IHC-P
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1:500-1:5,000
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IF-Cell
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1:500-1:1,000
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IP
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1-2μg/sample
Target
Function
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Background References
1. Wong RCB t al.itochondrial replacement in an iPSC model of Leber\'s hereditary optic neuropathy.Aging (Albany NY)9:1341-1350 (2017).
2. Ho SR al.NF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage.Proc Natl Acad Sci U S A 11:E2646-55 (2014).
Sequence Similarity
Belongs to the ku80 family.
Post-translational Modification
ADP-ribosylated by PARP3.; Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.; Sumoylated.; Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks.
Subcellular Location
Nucleus. Chromosome.
UNIPROT
Synonyms
86 kDa subunit of Ku antigen antibody
ATP dependent DNA helicase 2 subunit 2 antibody
ATP dependent DNA helicase II 80 kDa subunit antibody
ATP dependent DNA helicase II 86 Kd subunit antibody
ATP dependent DNA helicase II antibody
ATP-dependent DNA helicase 2 subunit 2 antibody
ATP-dependent DNA helicase II 80 kDa subunit antibody
CTC box binding factor 85 kDa antibody
CTC box-binding factor 85 kDa subunit antibody
CTC85 antibody
ExpandImages
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Western blot analysis of Ku80 on different cell lysate using anti-Ku80 antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: Hela
Lane 3: MCF-7
Lane 4: Human liver -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Ku80 antibody (R1511-17) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-17) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ku80 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Ku80 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ku80 antibody. Counter stained with hematoxylin.
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Ku80 was immunoprecipitated from 0.2 mg HeLa cell lysate with R1511-17 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using R1511-17 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: R1511-17 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of R1511-17 in HeLa cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 46 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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