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KLHDC3 Mouse Monoclonal Antibody [A8-E8]

Usd: 350

Specification

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Catalog# M1310-1

KLHDC3 Mouse Monoclonal Antibody [A8-E8]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Mouse

  • Human

Conjugation

  • unconjugated

Overview

Product Name

KLHDC3 Mouse Monoclonal Antibody [A8-E8]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human KLHDC3 aa 121-382 / 382.

Species Reactivity

Mouse, Human

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 43 kDa

Positive Control

Mouse testis tissue lysates, Hela, PC-3, human testis tissue, human ovary tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

A8-E8

RRID

AB_3073065

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:200-1:500

  • IF-Cell

  • 1:50-1:200

Target

Function

Peas is specifically expressed in testis, localized in pachytene spermatocyte cytoplasm and chromatin, and homologous to RAG2 raise a possibility that Peas might be involved in the meiotic recombination in testis.

Background References

1. "A novel testis-specific RAG2-like protein, Peas: its expression in pachytene spermatocyte cytoplasm and meiotic chromatin." Ohinata Y., Sutou S., Mitsui Y. FEBS Lett. 537:1-5(2003)

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q9BQ90 Human

Synonyms

dJ20C7.3 antibody

hPEAS antibody

kelch domain containing 3 antibody

Kelch domain-containing protein 3 antibody

KLDC3_HUMAN antibody

KLHDC3 antibody

PEAS antibody

Protein Peas antibody

RP1-20C7.3 antibody

Testis intracellular mediator protein antibody

Images

  • Immunocytochemistry analysis of Hela cells labeling KLHDC3 with Mouse anti-KLHDC3 antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-KLHDC3 antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling KLHDC3 with Mouse anti-KLHDC3 antibody (M1310-1) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-KLHDC3 antibody (M1310-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of PC-3 cells labeling KLHDC3 with Mouse anti-KLHDC3 antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-KLHDC3 antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of PC-3 cells labeling KLHDC3 with Mouse anti-KLHDC3 antibody (M1310-1) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-KLHDC3 antibody (M1310-1) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-KLHDC3 antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-KLHDC3 antibody (M1310-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1310-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human ovary tissue with Mouse anti-KLHDC3 antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ovary tissue with Mouse anti-KLHDC3 antibody (M1310-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1310-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-KLHDC3 antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1310-1" style="font-weight: bold;text-decoration: underline;">M1310-1</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-KLHDC3 antibody (M1310-1) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1310-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis on mouse testis tissue lysates using anti-KLHDC3 mouse mAb.

    Western blot analysis on mouse testis tissue lysates using anti-KLHDC3 mouse mAb.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"