KHSRP Mouse Monoclonal Antibody [A7-B0]
Catalog# EM1701-97
KHSRP Mouse Monoclonal Antibody [A7-B0]
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
KHSRP Mouse Monoclonal Antibody [A7-B0]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human KHSRP aa 625-674 / 711.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 73 kDa
Positive Control
Jurkat cell lysate, HT-29.
Conjugation
unconjugated
Clone Number
A7-B0
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgM
Purification Method
Protein L affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:50-1:100
Target
Function
Binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Background References
1. Min H et al. A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer. Genes Dev 11:1023-1036(1997).
2. Davis-Smyth T et al. The far upstream element-binding proteins comprise an ancient family of single-strand DNA-binding transactivators. J Biol Chem 271:31679-31687(1996).
Sequence Similarity
Belongs to the KHSRP family.
Tissue Specificity
Detected in neural and non-neural cell lines.
Post-translational Modification
Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.
Subcellular Location
Nucleus.
Synonyms
Far upstream element-binding protein 2 antibody
FBP2 antibody
FUBP2 antibody
FUBP2_HUMAN antibody
FUSE binding protein 2 antibody
FUSE-binding protein 2 antibody
KH type splicing regulatory protein (FUSE binding protein 2) antibody
KH type splicing regulatory protein antibody
KH type-splicing regulatory protein antibody
KHSRP antibody
ExpandImages
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Western blot analysis of KHSRP on Jurkat cell lysate using anti-KHSRP antibody at 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"