IRX2 Rabbit Polyclonal Antibody
Catalog# R1307-5
IRX2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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unconjugated
Overview
Product Name
IRX2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to Human IRX2 aa 422-471 / 471.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 49 kDa
Positive Control
Hela cell lysate, HepG2 cell lysate, SiHa cell lysate, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50-1:100
Target
Function
Iroquois-class homeodomain protein IRX-2, also known as Iroquois homeobox protein 2, is a protein that in humans is encoded by the IRX2 gene. IRX2 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos. IRX2 gene has been observed progressively downregulated in Human papillomavirus-positive neoplastic keratinocytes derived from uterine cervical preneoplastic lesions at different levels of malignancy. For this reason, IRX2 is likely to be associated with tumorigenesis and may be a potential prognostic marker for uterine cervical preneoplastic lesions progression.
Background References
1. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011)
2. "Cloning and chromosome mapping of human and chicken Iroquois (IRX) genes." Ogura K., Matsumoto K., Kuroiwa A., Isobe T., Otoguro T., Jurecic V., Baldini A., Matsuda Y., Ogura T. Cytogenet. Cell Genet. 92:320-325(2001
Sequence Similarity
Belongs to the TALE/IRO homeobox family.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Homeodomain protein IRXA2 antibody
Iroquois homeobox 2 antibody
Iroquois homeobox protein 2 antibody
Iroquois-class homeodomain protein irx-2 antibody
IRX 2 antibody
irx2 antibody
IRX2_HUMAN antibody
IrxA2 antibody
Images
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Western blot analysis of IRX2 on different lysates with Rabbit anti-IRX2 antibody (R1307-5) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: SiHa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-5) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunofluorescent staining of Hela cells using anti- IRX2rabbit polyclonal antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"