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IMP3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1512-10

IMP3 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

IMP3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human IMP-3 aa 352-579 / 579.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 64 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, THP-1 cell lysate, rat brain tissue, human placental tissue, mouse testis tissue, mouse brain tissue, HepG2.

Conjugation

unconjugated

RRID

AB_3073386

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB
human
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:400

  • FC

  • 1:50-1:100

Target

Function

IGF-II mRNA-binding proteins (IMPs) bind RNA and influence RNA synthesis and metabolism. IMP-1, also known as coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein (CRD-BP) and VICKZ1; IMP-2 (IMP2, VICKZ2, p62); and IMP-3 (KOC1, VICKZ3) contain a unique combination of RNA recognition motifs and four hnRNP K homology domains. IMP-1 is abundant in embryonal tissues and is expressed in 81% of colon cancers, 73% of sarcomas and 58.5% of breast cancers. It recognizes c-Myc, IGF-II and t mRNAs, and H19 RNA, and plays a major role in proliferation of K-562 cells by an IGF-II-dependent mechanism. IMP-2 binds the 5' UTR of IGF-II mRNA and influences tumor cell growth, in which IMP-2 is associated with apoptosis induced by tretinoin. IMP-3 knockdown by RNA interference decreases levels of IGF-II protein without affecting IGF-II, c-Myc, or β Actin mRNA and H19 RNA levels. IMP-3 is a marker for carcinomas and high-grade dysplastic lesions of pancreatic ductal epithelium.

Background References

1. Davidson, B. et al. VICKZ2 protein expression in ovarian serous carcinoma effusions is associated with poor survival. Human pathology. 45: 1520-8 (2014).

2. Rivera Vargas, T. et al. Post-transcriptional regulation of cyclins D1, D3 and G1 and proliferation of human cancer cells depend on IMP-3 nuclear localization. Oncogene (2013).

Sequence Similarity

Belongs to the RRM IMP/VICKZ family.

Tissue Specificity

Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: O00425 Human

SwissProt: Q9CPN8 Mouse

Entrez Gene: 312320 Rat

Synonyms

Cancer/testis antigen 98 antibody

CT98 antibody

DKFZp686F1078 antibody

hKOC antibody

IF2B3_HUMAN antibody

IGF II mRNA binding protein 3 antibody

IGF-II mRNA-binding protein 3 antibody

IGF2 mRNA binding protein 3 antibody

IGF2 mRNA-binding protein 3 antibody

IGF2BP3 antibody

Expand

Images

  • Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (<a href="/products/R1512-10" style="font-weight: bold;text-decoration: underline;">R1512-10</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: THP-1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 64 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1512-10" style="font-weight: bold;text-decoration: underline;">R1512-10</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (R1512-10) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: THP-1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 64 kDa
    Observed band size: 70 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (<a href="/products/R1512-10" style="font-weight: bold;text-decoration: underline;">R1512-10</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-IMP3 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 64 kDa<br />Observed band size: 64 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1512-10" style="font-weight: bold;text-decoration: underline;">R1512-10</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (R1512-10) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-IMP3 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

    Exposure time: 3 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-10) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placental tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placental tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-IMP3 antibody (<a href="/products/R1512-10" style="font-weight: bold;text-decoration: underline;">R1512-10</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1512-10" style="font-weight: bold;text-decoration: underline;">R1512-10</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-IMP3 antibody (R1512-10) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-10) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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